盐胁迫条件下甜高粱品种KF0680-1和KF0680-2的萌发及其α -淀粉酶表达研究

摘要

[Object] The aim was to study on germination and expression of a-amylase of KP0680-1 and KF0680-2 (cultivars of sweet sorghum) under salt stress. [ Method] In the research, KF0680-1 and KF0680-2, cultivars of sweet sorghum, were used to measure related indices of germination and expression of a-amylase to discuss effect of salt stress on two cultivars. [ Result ] Germination of sweet sorghum would be promoted if treated with salt in low concentration and inhibited if treated in high concentration. In the latter condition, roots and seedlings were smaller than that of control group in length, but expression of a-amylase in the two cultivars was promoted under proper salt stress. [ Conclusion ] The research indicates that KFD680-1 and KP0680-2 are inhibited in growth by salt stress and the latter is stronger in salt resistance than the former under salt in low concentration. In addition, expression of a-amylase could be promoted by salt in proper volume. The results provide references for selection of sweet sorghum which could be planted in northwestern areas.%[目的]研究2个甜高粱品种KF0680-1和KF0680-2在盐胁迫条件下的萌发特性及其α -淀粉酶表达.[方法]以甜高粱品种KF0680-1和KF0680-2为研究对象,对盐胁迫条件下甜高粱的萌发相关指标和α-淀粉酶的表达进行测定,探讨盐胁迫对2个甜高粱品种的影响.[结果]低浓度的盐胁迫对甜高粱的萌发有一定的促进作用,高浓度的盐胁迫则抑制了甜高粱萌发；在高浓度盐胁迫下根和幼苗的长度均小于水处理组,但适当的盐胁迫促进了2个品种α-淀粉酶的表达.[结论]该研究表明盐胁迫对KF0680-1和KF0680-2 2个甜高粱品种的生长均有抑制作用,但在低盐浓度条件下,KF0680-2比KF0680-1具有更好的抗盐性.同时适当的盐胁迫提高了种子中α-淀粉酶的表达量.该结果为选育出适宜西北地区种植的抗盐碱甜高粱品种提供了参考依据.