鹅绒委陵菜多糖对荷瘤小鼠细胞因子的影响

摘要

[ Objective ] To study the effects of polysaccharides from Potentilla anserine ( PAP) on the levels of interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α) from peripheral blood. [Method] The mice models with tumor S180 were established and treated with 50, 100 and 200 mg/kg PAP by gastric perfusion, respectively. The inhibition rate of S180 tumor was measured to investigate the antitumor activity in vivo of PAP. The levels of IL-2 and TNF-a in mice peripheral blood were determined with ELISA. [ Result ] The tumor inhibition rates were 46. 41 % , 52. 29% and 59.48% in different groups treated with 50,100 and 200 mg/kg PAP in vivo, respectively. Of these, the inhibition rate of CY and low-dose PAP was equal to that of medium-and high-dose PAP, significantly higher than that of cyclophosphamide. Three dose groups of PAP could significantly increase the content of IL-2 and TNF-a spleen index. [ Conclusion ] The compound PAP could improve the antitumor activity and immunological function of S180 tumor-bearing mice.%[目的]研究鹅绒委陵菜多糖(PAP)对荷瘤小鼠外周血细胞因子白介素-2(TL-2)和肿瘤坏死因子-α(TNF-α)的影响.[方法]建立肉瘤S180小鼠动物模型,灌胃给予50、100和200mg/kg剂量的PAP,以抑瘤率为指标,考察鹅绒委陵菜多糖的体内抗肿瘤活性,测定其抑瘤率；ELISA法测定荷瘤小鼠血清中白细胞介素-2(IL-2)和肿瘤坏死因子-α(TN-α)的水平.[结果]低、中、高3个剂量的PAP对S180荷瘤小鼠的抑瘤率分别为46.41％、52.29％和59.48％；其中CY和低剂量的多糖组合抑瘤率与中、高剂量的多糖抑瘤率相当,并明显高于单用环磷酰胺组的抑瘤率；低、中、高3个剂量的PAP能明显提高荷瘤小鼠血清中IL-2、TNF-α的水平.[结论]鹅绒委陵菜多糖具有提高荷瘤小鼠细胞免疫的作用.