EFFECTS OF EXOPOLYSACCHARIDE FRACTION (EPSF) FROM A CULTIVATED CORDYCEPS SINENSIS ON SPLENIC LYMPHOCYTE ACTIVITY OF H22 TUMOR BEARING MICE

摘要

In order to explore the effects of exopolysaccharide fraction (EPSF) from one of the anamorph strains of Cordyceps sinensis on splenic lymphocyte activity of tumor bearing mice, ICR mice were treated with EPSF for 7 days by intraperitoneally injection at doses of 15 mg/kg (low-dose), 30 mg/kg (mid-dose) and 60 mg/kg (high-dose) after H22 tumor cells were implanted. At the end of the experiments, the tumor weight of each mouse was measured. Proliferation ability and cytotoxicity of spleen lymphocytes were determined by MTT methods. Cytokme TNF - α and IFN - γ mRNA of spleen lymphocytes were assayed by RT - PCR. The results indicated that EPSF not only significantly inhibited the H22 tumor growth at doses of 30 mg/kg and 60 mg/kg (P＜0. 05), but also significantly enhanced host's spleen lymphocyte proliferation ability at all the three doses (P＜0. 001) and elevated cytotoxicity at dose of 60 mg/kg (P＜0. 01). EPSF also significantly elevated TNF - α and IFN - γ expression of splenic lymphocytes (P＜0. 01, 30 mg/kg; P＜0. 05, 60 mg/kg). This experimental finding suggests that EPSF could elevate the splenic lymphocytes' activity in H22 tumor bearing mice.