青蛤不同地理群体遗传结构差异的AFLP分析

摘要

[目的]探讨青蛤种群遗传多样性水平和遗传分化特征.[方法]采用AFLP技术对我国山东潍坊(WF)、江苏南通(NT)、浙江宁波(NB)、浙江温州(WZ)沿海共4个青蛤地理群体的遗传结构差异进行了分析.[结果]5对引物共得到261个位点,4个群体的多态位点比例平均为82.95％,其中WZ群体最高(86.97％),NT群体最低(78.93％)；4个群体Nei's基因多样性指数分别为0.2581、0.2526、0.2713和0.2776,香农多样性指数分别为0.3947、0.3830、0.4120和0.4214；群体遗传变异主要来自于群体内,占96.39％；WF和NT群体、NB和WZ群体遗传关系较近,聚类分析分别先聚在一起.[结论]研究的青蛤4个群体遗传基础较好,且尚未有明显的遗传分化.%[Objective] The aim was to discuss the genetic diversity level and classification of Cyclina sineusis. [Method] The genetic structure of four geographical populations from costal China of Weifang of Shandong (WF) , Nantong of Jiangsu (NT) , Ningbo of Zhejiang (NB) , and Wenzhou of Zhejiang (WZ) were analyzed by using AFLP Markers. [Result] The total 261 clear amplification bands were detected through five pairs of AFLP primer, the average rate of polimorphic loci was 82.95% , of which the greatest was WZ population(86. 97% ) and the lowest was NT population(78. 93% ). The gene diversity indexes of 4 populations of WF, NT, NB and WZ, the Nei were 0. 258 1, 0. 252 6, 0. 271 3 and 0.277 6, and Shannon's information index were 0. 394 7, 0. 383 0, 0.412 0 and 0.421 4. Genetic variations mainly came from individuals within populations (96. 39% ). WF and NT population, NB and WZ population were clustered together because of close genetic relation. [Conclusion] The genetic heredity of Cyclina sineusis. Was good and it showed indistinct heredity classification.