An Analysis of Genetic Diversity in Cyclina sinensis (Mollusca Bivalvia) of Four Geographical Populations Using AFLP Technique

摘要

This article preliminarily studied the genetic diversity in 109 individuals of Cyclina sinensis (Mollusca Bivalvia) of four geographical populations distributed around China's Bohai region based on amplified fragment length polymorphism, AFLP technique. Using 6 primer pairs including E-ACT/M-CGT，a total of 90 bands were detected of which 82 bands (91.11%) were polymorphic. The diversity index of each wild population was: TG 66.67%，DG 70%，HG 76.67% and BDH 83.33% while the Nei's genetic diversity index followed 0.2149，0.2162，0.2520 and 0.2895. Analysis suggested similar genetic diversity levels among the four populations in Bohai area and there was no significant difference (P>0.05). AMOVA analysis and UPGMA analysis conducted to the genetic differentiation degree of the four groups revealed an unnoticeable level of genetic diversity, and there was a certain degree of inter-population gene flow phenomenon. The genetic variations among the individuals within one population make up the main part of the total, which accounted for 86.02% total variance. The result above agreed with the conclusion of Y. Yuan et al. (2008) on the genetic structures of different geographical populations of Cyclina sinensis distributed in Yellow Sea and Bohai region based on ITS sequencing methods.