锦州地区猪传染性胸膜肺炎放线杆菌的分离与鉴定

摘要

[Objective] The research aimed to investigate the prevalence of porcine infectious pleuropneumonia in Jinzhou area. [ Method ] Five shares of diseased materials were colleted from some pig farms of Jinzhou area. The pathogens were isolated and identified by the culture test, biochemical test,satellite phenomenon examination,hematolysis test,CAMP test,drug sensitivity test and animal inoculation test. [ Result] The pathogens were Gram - negative short bacillus,with polymorphism and no spore. Five strains of porcine infectious actinobacillus pleuropneumonia were isolated and they all had satellite phenomenon and hematolysis phenomenon. CAMP test results showed that its hemolytic circle was strengthened by Staphylococcus aureus. Five isolated strains showed high sensitivity to cefradine, cephalosporin V and norfloxacin, but they were resistant to amoxycillin,streptomycin,penicillin and aureomycin. The animal inoculation test showed that the pathogen had high pathogenicity to rabbit. [Conclusion] The research could provide basis for the clinical treatment of porcine infectious pleuropneumonia.%[目的]调查锦州猪传染胸膜肺炎的流行情况.[方法]从锦州部分发病猪场采集病料5份,通过细菌的分离培养、生化试验、卫星现象观察,溶血试验、CAMP试验、药敏试验和动物接种试验对致病菌进行分离与鉴定.[结果]该病原菌为革兰氏阴性短小杆菌,无芽孢,具有多形性.分离到5株猪传染性胸膜肺炎放线杆菌,均有卫星现象和溶血现象.CAMP试验结果表明,金黄色葡萄球菌可增强其溶血圈.5株分离菌对头孢拉定、先锋V、氟哌酸高度敏感,而对阿莫西林、链霉素、青霉素、金霉素有耐药性.动物接种试验表明该致病菌对家兔具有较高的致病力.[结论]该研究可为猪传染性胸膜肺炎的临床治疗提供依据.