microRNA表达水平检测技术的研究进展

摘要

[目的]综述microRNA( miRNA)表达检测技术的研究进展,为miRNA表达研究及阐明其调控功能奠定基础.[方法]通过概述miRNA表达水平检测技术的原理、特点及进展,说明如何简便有效地检测其表达.[结果]miRNA是一类重要的非编码小RNA分子,调控一系列重要生命过程,其表达研究是研究其调控功能的重要途径,用于检测miRNA表达的方法主要有Northern blot、原位杂交(In Situ Hybridization,ISH)、表达谱芯片、RT-PCR以及克隆测序.[结论]该研究为简便有效地检测miRNA表达提供了方法,为阐明其对动植物重要生命过程的调控机理奠定了基础.%[Objective]The aim was to summarize the research progress of microKINA( miruNA) expression detection technique, to lay the foundation for miRNA expression study and it' s functional control. [Methods] Through summarizing the principles, characteristics and progress of miRNA expression level detection technique, to explain how to detect it' s expression simply and effectively. [Result] miRNA is an important class of small non-protein coding RNA, regulate and control a series of important life processes, it' s expression study is the important approach for the study of it' s control function, the detection method for miRNA expression are Northern blot, In Situ Hybridization, ISH, Expression Profile Chip, RT-PCR and cloning and sequencing. [Conclusion] The study provides methods for detecting miRNA expression simply and effectively, and lay the foundation for expounding it' s control mechanism for important life processes of animals and plants.