不同产地金银花与山银花中绿原酸含量的比较研究

摘要

[Objective] To compare the differences in the content of chlorogenic acid between Shandong Pingyi, Henan Fengqiu LONICERAE JAPONICAE FLOS and Hunan Longhui, Chongqing Xiushan, Chongqing Wulong, Chongqing Jiangjin LONICERAE FLOS. [Method] The content of chlorogenic acid in LONICERAE JAPONICAE FLOS and LONICERAE FLOS from different producing areas was determined by RP-HPLC. The separation was performed on a chromatographic column Platisil ODS (250 mm×4.6 mm, 5 μm) with the detection wavelength at 326 nm. Following the injection of sample solution, an elution with acetonitrile-0.2% phosphoric acid as mobile phase (17: 83,K/K) was initiated at a flow rate of 1.0 ml/min. [Result] The content of chlorogenic acid in Hunan Longhui, Chongqing Xiushan, Chongqing Wulong and Chongqing Jiangjin LONICERAE FLOS was 9. 03% , 8. 62% , 4. 51% and 3. 71% respectively, and that in Shandong Pingyi and Henan Fengqiu LONICERAE JAPONICAE FLOS was 4. 08% and 3. 79%. Of them, the content of chlorogenic acid was the highest in Chongqing Xiushan LONICERAE FLOS. [Conclusion] This method is reliable, stable and sensitive with high precision, strong resolving power and high recovery rate, and could be used for determining the content of chlorogenic acid in different producing areas of LONICERAE JAPONICAE FLOS and LONICERAE FLOS.%[目的]比较山东平邑、河南封丘的金银花与湖南隆回、重庆秀山、武隆、江津的山银花中绿原酸含量差别.[方法]采用HPLC测定不同产地金银花与山银花中绿原酸的含量.色谱条件:色谱柱Platisil ODS(250 mm×4.6 mm;5 μm),流动相为乙腈-浓度0.2％磷酸( 17:83,V/V),流速为1.0 ml/min,检测波长为326 nm.[结果]重庆秀山、重庆江津、重庆武隆和湖南隆回的山银花中绿原酸含量分别为9.03％、8.62％、4.51％和3.71％；山东平邑和河南封丘2个产地金银花中绿原酸的含量为4.08％和3.79％.重庆秀山的山银花中绿原酸含量明显高于其他几个产地的山银花中绿原酸含量,也高于山东、河南2个产地的金银花中绿原酸的含量.[结论]该提取方法可靠、稳定,检测方法灵敏、精密度高、分离度和回收率良好,可用于不同产地金银花与山银花中绿原酸的含量测定.