首页> 中文期刊> 《安徽农业科学》 >梭鱼养殖群体与自然群体的遗传结构研究

梭鱼养殖群体与自然群体的遗传结构研究

         

摘要

[目的]掌握渤海地区梭鱼种群的种质资源状况.[方法]应用随机扩增多态DNA(RAPD)技术,对梭鱼养殖群体与自然群体的遗传多样性进行了分析.[结果]从45个随机引物中选出20个扩增效果稳定的引物进行群体分析.其中,梭鱼自然群体共检出了204个扩增位点,多态位点136个,多态位点比例为66.7%;养殖群体中共检出了217个扩增位点,多态位点130个,多态位点比例为59.9%.[结论]目前梭鱼群体的遗传多样性较为丰富,人工养殖还未对其造成明显的影响.%[Objective] The research aimed to study the resources of Liza haematocheila population in Bohai Region. [Method] Using Random amplified polymorphic DNA (RAPD) , the genetic diversity of cultivated population and natural population of L. haematocheila was analyzed. [Result] 20 primers with stable amplification effects were selected from 45 random primers for population analysis. In natural population, 204 amplification sites were detected, and 136 sites were polymorphic and the percentage of polymorphic loci was 66.1%. In cultivated population, 217 amplification sites were detected, and 130 sites were polymorphic and the percentage of polymorphic loci was 59.9%. [Conclusion] At present, L haematocheila population has abundant genetic diversity and artificial cultivation hasn' t obvious effects.

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