[目的]对利用产黄青霉培养液的上清液生物合成纳米银的影响因素进行研究.[方法]利用紫外-可见光谱和投射电镜研究硝酸银起始浓度、pH值、光照和微波辐照等条件对合成反应速率及合成纳米银粒径的影响.[结果]硝酸银的最佳起始浓度为2 mmol/L；随着反应体系pH值的升高,反应速率随之加快,合成纳米银粒子的粒径变小；光照和微波辐照均能有效促进纳米银的合成.[结论]该研究为实现纳米银的可控合成提供了试验基础.%[Objective] The aim was to study the effects of experimental conditions on silver nanoparticles's biosynthesis by culture supernatant of Penicillium chrysogenum. [ Method] The effects of initial silver nitrate concentration,pH value, visible-light emission and microwave irradiation on the reaction rate and size of synthetic: silver nanoparticles were studied by using UV-vis spectra and transmission electron microscopy. [Result] The optimal initial silver nitrate concentration was 2 mmol/L; the reaction rate was increased with the increase of pH value in the reaction system , but the particle size was decreased; both visible-light emission and microwave irradiation could promote the synthesis of silver nanoparticles effectively. [Conclusion] This research provided a test basis for the realization of controllable synthesis of silver nanoparticles.
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