碱提麻竹竹秆多糖的相对分子质量测定及单糖组成分析

摘要

[目的]获得更多的竹资源信息。[方法]采用碱法提取麻竹竹秆多糖，提取物用Sevag法去蛋白，95％乙醇醇沉，透析，DEAE－52纤维素柱分离纯化，得到多糖DLP1和DLP2，并对多糖进行了相对分子质量测定及单糖组成分析。[结果]测得多糖DLP1和DLP2的相对分子质量分别为61500和1780000 u；经气象色谱测定DLP1由岩藻糖、鼠李糖、阿拉伯糖、葡萄糖和半乳糖组成，其质量比为111．25∶17．81∶1∶12．43∶2．43；DLP2由岩藻糖、鼠李糖、阿拉伯糖、葡萄糖、半乳糖和甘露醇组成，其质量比为198．22∶3．27∶9．16∶25．06∶10．96∶1。[结论]该实验为麻竹多糖研究打下基础，为竹资源的深度开发提供科学依据。%Objective] To know more about bamboo.[Method] The polysaccharides in the pole of D.latiflorus were extracted by alkali solu-tion.The proteins were removed by Sevag agent from extraction.The polysaccharides in extraction were precipitated by alcohol of 95%, and then dialyzed by dialysis bag, separated by DEAE-52 cellulose column.Polysaccharides of DLP1 and DLP2 were obtained, and their relative molecular weights were measured and monosaccharide compositions were analyzed .[ Result] The relative molecular weights of polysaccharides DLP1 and DLP2 were detected to be 61 500 and 1 780 000 u.Their monosaccharide composition was determined by gas chromatography. DLP1 was composed of fucose (Fuc), rhamnose (Rha), alabinose (Ala), glucose (Glu) and galactose (Gal) at a quality ratio of 111.25∶17.81∶1∶12.43∶2.43.DLP2 was composed of fucose (Fuc), rhamnose (Rha), alabinose (Ala), glucose (Glu), galactose (Gal) and mannitol (Mnt) at a quality ratio of 198.22∶3.27∶9.16∶25.06∶10.96∶1.[Conclusion] The work laid a good foundation for studying on poly-saccharides of D.latiflorus, and provided scientific basis for further development of bamboo resource .