闪式提取法提取海燕浸膏工艺研究

摘要

Objective] Asterina pectinifera contains a large quantity of chemical components with biological activity, and studies on extraction of saponin from A.pectinifera hasgreat significance to utilization of A.pectinifera.[ Method] Saponin was extracted from A.pectinifera by using flash-type extractor JHBE-50T, and the effects of ethanol concentration, extraction time and solid-liquid ratio on the yield of saponin from A.pectinifera were analyzed.[Result] The factors affecting the extraction rate of saponin from A.pectinifera were shown as follows:extraction time, solid-liquid ratio, and ethanol concentration.The optimum extraction conditions were 75% ethanol, solid-liquid ratio 1∶30 g/mL, and 90 s of extraction. [Conclusion] Saponin extracted from A.pectinifera by using flash-type extractor had high yield, which can lay foundation for the extraction of chemical components with biological activity from A.pectinifera.%[目的]海燕中含有大量的具有生物活性的化学成分，研究海燕浸膏的提取，对利用海燕资源具有重要意义。[方法]利用闪式提取法提取海燕浸膏，考察了乙醇浓度、提取时间、料液比对海燕浸膏提取率的影响。[结果]闪式提取法制备海燕浸膏，影响海燕总皂苷含量的因素大小依次为闪式提取时间、料液比、乙醇质量分数，最优提取条件为75％的乙醇，料液比为1∶30 g／mL，闪式提取90 s。[结论]利用闪式提取法制备海燕浸膏获得了较好的效果，为进一步从海燕中获得生物活性化学成分奠定了基础。