[Objective] The aim was to optimize the transformation conditions of organic selenium by Phaffia rhodozyma.[Method] The effect of selenium addition, culture time and liquid loading on selenium accumulation in Phaffia rhodozyma was studied by orthogonal experiment .[ Re-sult ] The optimum medium for selenium enrichment was PDA medium , and the adding method was added in two times .Under the condition of se-lenium content at 20 mg/L and the liquid loading at 80 mL/500 mL incubating for 30 h, the biomass and transformation rate of organic selenium could reach 106.2 g/L and 63.2%, respectively.[Conclusion] The results can be regarded as the reference for further research on selenium en -richment in Phaffia rhodozyma.%[目的]优化红发夫酵母(Phaffia rhodozyma)有机硒转化条件.[方法]通过正交试验研究硒添加量、培养时间和装液量对红发夫酵母富硒的影响.[结果]红发夫酵母富硒的最适培养基为PDA培养基,亚硒酸钠的添加方式为分2次添加,在硒添加量20 mg/L、培养时间30 h、装液量80 mL/500 mL的条件下,红发夫酵母的生物量达10.62 g/L,有机硒转化率达63.2%.[结论]研究结果为红发夫酵母富硒的进一步研究提供了参考.
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