[目的]研究骨形态发生蛋白受体IB(Bone morphogenetic protein receptor IB,BMPR-IB)基因多态性与湖羊产羔性能的关系.[方法]采用PCR扩增、酶切和序列测定等方法对湖羊BMPR-IB基因型进行鉴定,并分析BMPR-IB基因型与湖羊产羔数的关系.[结果]共检测出3种基因型,并将其分别定义为AA、AG、GG.测序结果表明,等位基因A与G相比发生了1处碱基突变(A746G).BMPR-IB基因在湖羊中存在多态性(AA、AG和GG);湖羊中A等位基因的频率为0.032,G等位基因的频率为0.968.AA、AG和GG基因型频率分别为0.006、0.053和0.941.AA、AG和GG基因型湖羊的平均产羔数分别为1.25、1.81和1.96.[结论]BMPR-IB基因可能是湖羊多胎性能的主效基因,可用于对湖羊产羔数的选择.%[Objective]To study the relationship between the polymorphism of bone morphogenetic protein receptor IB(BMPR-IB) gene and the lambing performance of Hu Sheep. [ Method] The genotypes of BMPR-IB gene was identified by the methods of PCR amplification,enzyme digestion and sequencing. And the relationship between the genotypes of BMPR-IB and litter size of Hu Sheep was analyzed. [ Result] Three genotypes were detected, and defined as AA, AG, GG, respectively. The sequencing results showed that the allele A and G had a base muta-tion (A746G), and there was BMPR-IB gene's polymorphism (AA, AG and GG) in Hu Sheep. The frequency of A allele in Hu Sheep was 0. 032, the frequency of G allele was 0. 968. The frequencies of AA,AG and GG genotype were 0. 006, 0. 053 and 0. 941,respectively. The average litter size of Hu Sheep with AA, AG and GG genotypes were 1. 25, 1. 81 and 1. 96,respectively. [Conclusion]BMPR-IB gene might be the major gene for the prolificacy of Hu Sheep, it could be used to select litter size of Hu Sheep.
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