[Objective] To discuss the optimum conditions for large-scale cultivation of eicosapentaenoic acid(EPA) by using microalgae.[Method] The effects of temperature on ENN1001A growth and EPA yield were studied.The effects of liquid level on the temperature were analyzed.The large-scale production of EPA was expounded.[Result] EPA yield in May and September were 738 and 786 mg/(m2· d)respectively.And the average annual production was 666 mg/(m2 ·d),which was far higher than the reported data [516 mg/(m2 ·d)].The content of EPA and the average temperature of algae medium had consistent changes and showed the changing trend of first decrease and then increase in total culture period.[Conclusion] The research results could lay the foundation for the large-scale cultivation of EPA.%[目的]探讨微藻规模化生产二十碳五烯酸(EPA)的最佳条件.[方法]研究了温度对ENN1001A生长及EPA产量的影响,分析了液位对温度的影响,阐述了EPA的规模化生产工艺.[结果]5月和9月EPA产量最高,分别达到738和786 mg/(m2·d),全年平均产量为666 mg/(m2·d),远高于文献报道的516 g/(m2 ·d).全养殖季EPA与藻液平均温度变化相一致,整体上呈先下降后上升的变化趋势.[结论]研究结果可为EPA的规模化生产奠定基础.
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