氯、锌离子对猪精子钙通道和若干酶活调控作用的研究

摘要

The regulating and controlling roles of chloride and zinc ions to swine sperm calcium channel and their mechanism were studied.Semen from healthy boar were filtered with gauze and divided into three groups,each group having 6 repeats.The group 1 semen was not added anything;the group 2 and 3 semen were added NaCl and ZnSO 4 solution respectively.Being mixed and placed 2.5 h,various parameters of 3 experimental semen were measured.Experimental results showed that：（1） supplementation of optimum chlorine ions or zinc ions in semen could stimulate migration of calcium ions from Seminal plasm to sperm,thus calcium ion concentration in sperm raised significantly（P 〈 0.05）,calcium ion concentration in Seminal plasm decreasesd slightly;（2） supplementation of chlorine or zinc ions in semen could strengthen sperm motility highly significantly（P 〈 0.01）;（3） after supplementation of zinc ions in semen,the activities of AKP,CA and AC and the concentration of cAMP in sperm increased highly significantly（P 〈 0.01）,the activities of AKP and AC in seminal plasm raised highly significantly（P 〈 0.01）,the concentration of cAMP in seminal plasm increased significantly（P 〈 0.05）;（4） after supplementation of chlorine ions in semen,the activities of AKP and AC in seminal plasm raised highly significantly（P 〈 0.01）,the concentration of cAMP in Seminal l plasm increased significantly（P 〉 0.05）.These results suggested that supplementation of optimum zinc or chlorine ions in boar semen may up-regulate the function of sperm calcium channel,and can improve the activities of AKP,CA and AC and the concentration of cAMP in semen,strengthen sperm motility.%研究了氯离子、锌离子等对猪精子钙通道的调控作用及其机理。将由健康公猪采得的精液经纱布过滤后分为3组,每组6个重复。第1组精液不加任何物质,第2、3组精液分别加氯化钠液和硫酸锌液,混匀并静置2.5h后,观测精子活力和精子、精清中离子含量与酶活。结果表明：（1）在猪精液中分别加适量的氯离子、锌离子,能刺激精清中钙离子向精子中转移,使得精子内钙离子含量显著地（P〈0.05）增加,精清中钙离子有一定程度的（P〉0.05）减少;（2）加锌或加氯离子,精子活力极显著（P〈0.01）地增强;（3）加锌离子组,精子内碱性磷酸酶、碳酸酐酶、腺苷酸环化酶活性极显著地（P〈0.01）提高,cAMP含量极显著地（P〈0.01）增多;精清内碱性磷酸酶、腺苷酸环化酶活性极显著地（P〈0.01）提高,cAMP含量显著地（P〈0.05）增多;（4）加氯离子组,精清内碱性磷酸酶、腺苷酸环化酶活性极显著地（P〈0.01）提高,精清内cAMP含量显著地（P〈0.05）增多;但精子内碳酸酐酶、腺苷酸环化酶活性和cAMP含量都无显著的（P〉0.05）变化。上述结果表明：猪精液加适量锌离子或氯离子,对精子细胞钙通道功能可能都有上调作用,并能提高精液中碱性磷酸酶、腺苷酸环化酶、碳酸酐酶活性,增加精液中cAMP含量,增强精子活力。