红掌愈伤组织诱导及再生体系的建立

摘要

In Anthurium varieties "pink champion" of leaves, petioles, pedicels and bracts of explant material, Anthurium callus induction and plant regeneration were studied to establish the Anthurium efficient regeneration system. Explore different explants, different sterilization time for callus induction; different concentrations of hormone combinations buds, rooting of. The results showed that: with 0.1%HgCl, leaves, petioles, pedicels and bracts sterilized optimum sterilization times were 8,12,12,10 min; petiole callus induction is the best explant; buds suitable formulations induced MS + 6-BA0.8 mg / L + NAA0.2 mg / L; rooting most suitable formula for the MS + charcoal 1.0 g / L + NAA0.5 mg / L.%以红掌品种“粉冠军”的叶片、叶柄、花梗及苞片为外植体材料，开展红掌愈伤组织诱导及植株再生研究，建立红掌高效再生体系，探讨不同外植体、不同灭菌时间对愈伤组织的诱导效果；不同质量浓度激素组合对芽诱导及试管苗生根的影响.结果表明：用0.1% HgCl2对叶片、叶柄、花梗及苞片进行灭菌，最佳灭菌时间分别为8、12、12、10 min；叶柄是愈伤组织诱导的最佳外植体；不定芽诱导适宜配方 MS+6-BA0.8 mg/L+NAA0.2 mg/L；试管苗生根最适宜配方为MS+活性炭1.0 g/L+NAA0.5 mg/L .