The pot experiment for single-factor with diesel oil polluted soil and the pot experiment for three-factor orthogonal with sawdust-ammonium nitrate-monopotassium phosphate under diesel oil polluted soil with salt stress, were performed to analyze the activity of antioxidant enzymes and chlorophyll content in Lolium perenne seedlings, and to explore the physiological response of L. perenne seedlings under diesel oil polluted soil and its regulations. The results showed that, soil diesel pollution significantly decreased the biomass. Compared with control, activity of superoxide dismutases(SOD)in leaf decreased significantly at 0.3% and 0.9% soil diesel pollution, peroxidases (POD) and catalase(CAT) in leaf decreased significantly at 0 . 6% and 0 . 9% soil diesel pollution , the root SOD activity increased significantly at 0.9%diesel concentration while the root POD activity decreased significantly at 0.6%and 0.9%soil diesel pollution. As for the salinity soil polluted by diesel oil, the activity of POD and CAT in leaf increased significantly at 10%volume fraction of sawdust, and the content of chlorophyll a and chlorophyll b increased significantly as well. Meanwhile, chlorophyll a and chlorophyll b content increased significantly at 0.3 g·kg -1 amount of ammonium nitrate. Thereby, sawdust and ammonium nitrate addition could effectively improve physiological metabolic of L. perenne seedlings.%开展了土壤柴油污染的单因素盆栽实验和柴油污染盐渍化土壤中添加锯末-硝酸铵-磷酸二氢钾的三因素正交盆栽实验,对黑麦草幼苗抗氧化酶活性和叶绿素含量进行了分析,探究了柴油污染土壤中黑麦草幼苗的生理变化与调节.结果表明,土壤柴油污染显著减小了黑麦草幼苗生物量,与对照相比,叶SOD活性在柴油浓度0.3%和0.9%时显著降低,POD和CAT活性在0.6%和0.9%柴油浓度下显著降低;根SOD活性在0.9%柴油浓度下显著增大,POD活性在0.6%和0.9%柴油浓度下显著下降.受柴油污染的盐渍化土壤,施加锯末体积分数为10%时,黑麦草幼苗叶POD和CAT活性显著增强,叶绿素a和叶绿素b含量显著增加;施氮量为0.3 g·kg-1土时,黑麦草幼苗叶绿素a和叶绿素b含量显著增加.可见,土壤受柴油污染时,添加锯末和硝酸铵可有效调节黑麦草幼苗的生理代谢.
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