In order to find stronger linked microsatellite markers, 9 polymorphic EST-SSR loci were selected in German Merino grading crossing offspring F2 with Chinese Merino sheep, then the genetic polymorphism of group and the correlation between microsatellite markers and wool traits were analyzed. The results showed that the loci had rich polymorphism and 39 alleles was detected among 9 loci in the group and 6 of them had significance differences between genotypes. There were 5 loci related with natural length, 4 loci related with stretched length and 3 loci related with the diameter. The results of F test for the relativity of EST-SSR markers with wool traits showed that 33176918 and 33176988 loci had significant genetic effects in the natural wool length and average diameter, respectively (P<0.05). The study suggests that 33176918 locus has significant marker effect on natural length and CD genotype is the favorable genotype; 33176988 locus has significant marker effect on fibre diameter and BC genotype is the favorable genotype. These two loci probable have high linkage with genetic locus of wool traits.%为了筛选与绵羊(Ovis aries)毛性状基因座连锁更强的分子标记,本研究选择9个多态性丰富的绵羊皮肤源EST-SSR位点,检测了德国美利奴羊与中国美利奴羊级进杂交F2代群体的遗传多态性,并分析了标记与毛性状间的关联性.分析结果显示,所选的9个EST-SSR位点在供试群体中共检测到39个等位基因,多态性丰富,其中的6个位点基因型间存在显著差异；其中,与自然长度关联的位点有5个,与伸直长度关联的位点有4个,与纤维直径关联的位点有3个.6个EST-SSR位点基因型间存在显著差异；关联性标记对羊毛性状相关效应的F检验结果表明:33176918位点和33176988位点分别对羊毛自然长度、纤维平均直径具有显著的遗传效应(P＜0.05).研究结果提示,与羊毛性状相关的6个位点中,33176918号位点对羊毛的自然长度有较大的标记效应,该位点的CD基因型是羊毛自然长度的有利基因型；33176988号位点对羊毛的纤维直径有显著地标记效应,该位点的BC基因型是纤维直径的有利基因型.这两个位点可能与控制毛形状的基因座紧密连锁.
展开▼
