目的：评估睾酮（Testosterone）联合芳香化酶抑制剂阿那曲唑及单用睾酮对雄激素受体（Androgen receptor，AR）阳性的乳腺癌细胞 MDA - MB -231（ER -/ AR +）作用的效果。方法：配置不同浓度的药物，CCK -8检测药物作用不同时间后细胞增殖抑制率，流式细胞凋亡分析试剂盒检测药物对细胞凋亡影响，荧光定量 PCR 法检测不同浓度药物作用下 MDA - MB -231细胞中 hsa- miRNA -30e 表达情况。结果：体外实验中阿那曲唑单药及低浓度睾酮对 MDA - MB -231细胞起到促增殖作用，高浓度睾酮及联合用药组会抑制 MDA - MB -231细胞增殖作用，并且联合用药组较睾酮单药组增殖抑制率（P ﹤0.05）及细胞凋亡率高（P ﹤0.01）；分析72 h 后不同药物浓度下 hsa - miRNA -30e 表达情况后显示，睾酮及联合用药组较阿那曲唑单药组可以明显降低其表达水平（P﹤0.01）。结论：睾酮联合芳香化酶抑制剂对 AR 阳性乳腺癌细胞具有较强的抑制作用，并且其作用机制可能通过 miRNA 调节途径发挥作用。%Objective To evaluate the inhibitory effects of testosterone combined with anastrozole on breast cancer cells MDA - MB -231 (ER - / AR + ). Method The different concentrations of drugs containing testosterone,anastrozole and combined drugs had been prepared with dimethyl sulfoxide. Perform CCK -8 and flowed cytometric detection with Annexin V/ PI double dyeing methods to examine the prolifera-tion and apoptosis of cells respectively. The expression of hsa - miRNA -30e under the different concentrations of drugs in 72 h had been de-tected through real - time PCR. Results In vivo experiment testosterone with low concentration and anastrozole promoted proliferation of MDA- MB -231 cells,but testosterone with high concentration and combination drugs could suppress the cells proliferation in 72 h. Testosterone and combination drugs had also significantly reduced the expression levels of hsa - miRNA - 30e than the anastrozole group in 72 h(P ﹤0. 01). Conclusion Testosterone combined with anastrozole have stronger inhibitory effect on AR positive breast cancer cells,and the mecha-nism of drugs action may be connected with regulation of miRNA.
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