银莱汤对食积复合FM1流感病毒感染小鼠黏膜免疫的影响

摘要

目的 探讨银莱汤对食积复合FM1流感病毒感染小鼠黏膜免疫的影响,为银莱汤药效机理的研究提供实验依据.方法 雄性昆明小鼠90只,随机分为正常组、感染组、食积感染组、银莱汤组高中低剂量组、双黄连组、小儿化食丸组、利巴韦林组.食积感染组及各治疗组均采用喂饲高蛋白、高热量饲料结合52%牛奶溶液灌胃的方法制作食积动物模型,感染则采用FM1流感病毒滴鼻处理,并予相应的治疗药物或生理盐水灌胃.取肺、结肠组织匀浆,采用ELISA法检测肺、结肠组织中sIgA、IL-10、TNF-α及INF-γ的含量.结果 与正常组相比,感染组和食积感染组结肠组织中sIgA、IL-10及TNF-α水平下降;肺组织中sIgA、INF-γ水平明显下降(P<0.05),IL-10、TNF-α水平升高.与食积感染组比较,银莱汤中剂量组肺及结肠组织的sIgA、IL-10水平升高较明显(P<0.05),而银莱汤各治疗组肺组织中的sIgA、IL-10水平升高较明显,TNF-α水平降低,而INF-γ在一定程度上升高,但无统计学意义.银莱汤各治疗组肺组织中的sIgA、IFN-γ较正常组明显降低(P<0.05).银莱汤各治疗组与阳性给药组对比,肺、肠组织中sIgA无统计学差异,肺、肠组织中IL-10明显升高(P<0.05),肺、肠组织中TNF-α明显降低(P<0.05),肺组织中的INF-γ也明显降低(P<0.05).结论 银莱汤可以通过促进肺、肠黏膜sIgA的分泌,调节IL-10、TNF-α、INF-γ的合成,从而调控小鼠的黏膜免疫功能,进而发挥其抗病毒的疗效.%Objective To explore the influence ofYinlai decoction on mucosal immunity in mice with dyspepsia combined with FM1 influenza virus infection, and identify the efficacy ofYinlai decoction and its potential mechanism.Methods 90 male mice were randomly allocated into normal decoction, infection decoction, infection and dyspepsia decoction, high, middle and low doseYinlai decoction ,Shuanghuanglian decoction,Xiaoer huashiwan decoction, ribavirin decoction. A diet-induced mice model with high fat and high protein diet combined with 52% milk solution were built in infection and dyspepsia decoction and all the treating decoction to build models of dyspepsia animal, and the mice were infected by FM1 influenza virus via nose in those decoction. The level of secreted immunoglobulin A (sIgA), interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α) and interferon-γ(INF-γ) of lung and colon tissue homogenate by ELISA detection.Results Compared with the normal decoction, the levels of sIgA, IL-10 and TNF-α in colon tissue decreased, the levels of sIgA, INF-γ in lung tissue decreased significantly (P<0.05), the levels of IL-10, TNF-α in lung tissue were increased in infection decoction as well as infection and dyspepsia compound decoction. Compared with infection and dyspepsia compound decoction, the levels of sIgA, IL-10 and TNF-α in colon tissue were increased significantly (P<0.05) inYinlai decoction decoction of middle, the levels of sIgA, IL-10 in lung tissue elevated significantly in each dose ofYinlai decoction decoction. Compared with the normal decoction, the levels of sIgA, INF-γ in lung tissue were decreased significantly (P<0.05). compared with the treating decoction, the levels of sIgA were not statistically different, the levels of IL-10 elevated significantly(P<0.05), the levels of TNF-α decreased significantly (P<0.05) in lung and colon tissue of each dose ofYinlai decoction decoction, the levels of INF-γ decreased significantly (P<0.05) in lung tissue.ConclusionYinlai decoction can promote the secretion of sIgA in lung and colon tissue, regulate the secretion of IL-10, TNF-α, INF-γ, thus regulate mucosal immune function of mice, and then achieve an anti-viral effect.