清肠止泻散对轮状病毒模型鼠血清TNF-α、IL-1β 水平及粪便Na+、糖含量的影响

摘要

目的 研究清肠止泻散对轮状病毒(RV)模型小鼠血清肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)含量及粪便Na+、糖含量的影响.方法 将40只SPF级昆明小鼠随机分为4组:空白组、模型组、清肠止泻散组、病毒唑组,每组10只.通过对实验小鼠造模、给药、取材,观察各组小鼠病死率、平均稀便级、轮状病毒抗原阳性率;检测小鼠粪便Na+、糖含量,用ELISA方法检测各组小鼠血清TNF-α、IL-1β 含量的变化.结果 清肠止泻散组死亡数低于模型组(P<0.05);治疗后清肠止泻散组轮状病毒检测阳性数低于模型组(P<0.05);治疗后清肠止泻散组平均稀便级低于模型组(P<0.05);清肠止泻散组Na+、糖含量低于模型组(P<0.01);与正常组比较,模型组血清TNF-α、IL-1β 在血清中含量明显升高(P<0.01,P<0.05);与模型组比较,清肠止泻散组可明显降低TNF-α、IL-1β含量(P<0.01).结论 清肠止泻散组对轮状病毒感染小鼠有一定的治疗作用,可缓解腹泻、降低病死率、病毒阳性数,可提高小肠对Na+、糖的吸收,可调节血清TNF-α、IL-1β 水平.%Objective To study the effect of Qingchang Zhixie powder on tumour necrosis factor-α(TNF-α), interleukin-1β(IL-1β) and feces Na+, glucose content on mice with rotavirus. Methods Forty SPF rats were randomly divided four groups: normal group,model group,Qingchang Zhixie powder group, ribavirin group, ten rats in each group. By means of experiment mice of making mode, givong medicine and taking ingredients. Occurrence of diarrhea and death rate was detected. The mortality rate of each group, average stool level, rotavirus antigen positive rate were observed; Detection of Na+, sugar content in mouse feces, ELISA method was used to detect the changes of serum levels of TNF-α and IL-1. Results The death rate in Qingchang Zhixie powder group was lower than that in model group(P<0.05); After treatment, the positive number of rotavirus in Qingchang Zhixie powder group was lower than that of model group (P<0.05); After treatment, the average level of stools in Qingchang Zhixie powder was lower than that of model group (P<0.05); Na+, sugar content in Qingchang Zhixie powder group was lower than the model group (P<0.01); Compared with normal group, the content of TNF-α, IL-1βin serum were obviously increased, in model group (P<0.01, P<0.05); Compared with model group, Qingchang Zhixie powder group could significantly reduce the content of TNF-α,IL-1β in serum (P<0.01). Conclusion Qingchang Zhixie powder is effective in treating rotavirus mice to certain extents,which can relieve the diarrhea, reduce mortality, counter rotavirus, promote the intestine to absorb Na+, glucose and regulate the TNF-α, IL-1βlevel of rotavirus mice.