GC法测定天然冰片中的d-龙脑和樟脑

摘要

GC-method was be used to determine the d-borneol and camphor in the natural borneol. The analytical column is CP-WAX capillary column<30 m×0.32 mm×0.5 μm>; temperature program was set as: heating rate with 5 ℃/min from the initial temperature 50 ℃to 100 ℃then keeping the temperature for 1 min, and warming up quickly to 180 ℃ with a rate of 10 ℃/min then keeping 5 min. Injection velocity ratio was 5∶1; FID detector temperature was 250 ℃; inlet temperature was 200 ℃. High purity nitrogen was as carried gas with the volume flow 2 mL/min; air volume flow was 300 mL/min; hydrogen volume flow was 30 mL/min; the injection volume was 1 μL. The method has some advantages like simple and accurate operation, good precision and short analysis time, it can provide references for determining and controlling the quality of natural bornel.%采用GC法测定天然冰片中的d-龙脑和樟脑的含量。分析柱为CP-WAX毛细管柱<30 m×0.32 mm×0.5μm>；升温程序为50℃，以5℃/min升温到100℃，保持1 min，再以10℃/min快速升温到180℃，保持5 min，进样流速比5∶1；FID检测器温度为250℃；进样口温度为200℃，载气为高纯氮气，体积流量为2 mL/min；空气体积流量为300 mL/min；氢气体积流量为30 mL/min；进样量为1μL。本方法操作简便、准确，精密度、分离度良好、分析时间短，为天然冰片的测定及其质量控制提供参考。