结核分枝杆菌HspX基因真核表达载体构建

摘要

In order to construct a recombinant plasmid carrying enhanced green fluorescent protein (EGFP)and Mycobacterium tuberculosis HspX gene,HspX gene was amplified by PCR and inserted into a multiple cloning site of eukaryotic expression vector pEGFP-N1. The results shows HspX eukaryotic expression vector labeled with FLAG tag was successfully constructed as demonstrated by double enzyme digestion and DNA sequencing. The conclusion is that the eukaryotic expression plasmid pEGFP-N1-HspX has been constructed successfully.%为构建结核分枝杆菌HspX基因与增强型绿色荧光蛋白（EGFP）融合基因的真核表达载体，文章采用PCR法扩增HspX基因，定向插入到质粒pEGFP-N1的多克隆位点。结果显示，融合基因真核表达载体，经双酶切鉴定、基因测序鉴定克隆片段大小和序列正确。文章成功构建了pEGFP-N1-HspX融合基因真核表达载体。