目的 探索131I-herceptin对高表达Her-2基因的乳腺癌细胞株的生物学作用.方法 MTT 比色法检测131I-herceptin对表达不同水平Her-2基因的三株乳腺癌细胞株SK-BR-3、MDA-MB-231、MCF-7的抑制作用,流式细胞术测量各干预组的DNA倍体和凋亡率.结果 131I-herceptin对SK-BR-3细胞的抑制作用明显强于对MDA-MB-231、MCF-7细胞的抑制作用(P＜0.05、P＜0.01),并且明显强于131I组、herceptin组以及131I+herceptin组(P＜0.05);131I-herceptin组的凋亡率(37.71%)明显高于131I+herceptin组(14.61%)(P＜0.05)、herceptin组(3.37%)和131I组(0.93%)(P＜0.01).各干预组都出现细胞周期的阻滞,131I-herceptin组最明显(73.19%).结论 131I-herceptin 对高表达Her-2基因的人乳腺癌细胞株SK-BR-3有明显的抑制和杀伤作用.%Objective To explore the biologic response of breast cancer cell lines highly expressing Her-2 gene to 131 I-herceptin. Methods The inhibitory effect of 131 I-herceptin on SK-BR-3,MDA-MB-231 and MCF-7 cell lines expressing different levels of Her-2 gene was evaluated with MTT assay. The DNA ploid and cell apoptosis rate of the intervention groups were evaluated by flow cytometery. Results The inhibitory effect of 131 I-herceptin on SK-BR-3 cells was significantly higher than that on MDA-MB-231 orMCF-7 cells(P＜0. 05),which was significantly higher than that of 131 Ⅰ group, herceptin group and 131Ⅰ＋ herceptin group as well (P＜ 0. 05). The apoptosis rate of 131I-herceptin group(37. 71＜) was significantly higher than that of 131Ⅰ group (0. 93％), herceptin group (3. 37％) or 131 I+ herceptin group(14. 61％)(P＜0. 01). A significant cell cycle blockage was seen in all intervention groups, especially in 131 I-herceptin group(73. 19 ％). Conclusion 131 I-herceptin has significant inhibiting and killing effect on human breast cancer cell line SK-BR-3 expressing high level of Her-2 gene.
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