藤黄酸对胃腺癌细胞株AGS凋亡的影响

摘要

Objective To investigate the effect of gambogic acid(GA) on proliferation and apoptosis of human gastric adenocarcinoma cell line AGS. Methods After AGS cells were treated with different concentrations of GA 0, 0.4, 0. 8, 1. 2, 1. 6, 2. 0, 2. 4 μmol/L, the inhibition rate of cell proliferation was measured by MTT assay, the apoptosis of AGS cells was analyzed by flow cytometry,and the protein expressions of B cell lymphoma/leukemia-2 (Bcl-2) and Bci-associated X protein(Bax) were detected by Western blot Results GA could inhibit cell proliferation,promote cell apoptosis,down-regulate protein expression of Bcl-2 and up-regulate protein expression of Bax in a dose-dependent manner. Moreover, the IC50 value was 4. 05 μg/ml. Conclusion GA can significantly inhibit proliferation and enhance apoptosis of AGS cells, which may be related to the expressions of Bcl-2 and Bax.%目的 探讨藤黄酸对人胃腺癌细胞株AGS的增殖和凋亡作用.方法 用不同浓度藤黄酸处理AGS细胞后,MTT法测定AGS细胞的增殖活性,流式细胞术分析藤黄酸诱导AGS细胞凋亡,Western blot法检测B细胞淋巴瘤-白血病2(Bcl-2)和Bcl相关X蛋白(Bax)表达的变化.结果 藤黄酸能剂量依赖性地抑制AGS细胞增殖、诱导AGS细胞凋亡、下调Bcl-2蛋白表达、上调Bax蛋白表达；24 h半数抑制浓度(IC50)为0.9μmol/L.结论 藤黄酸能明显抑制胃癌细胞株AGS的增殖,诱导AGS细胞凋亡.其促凋亡作用可能与Bcl-2、Bax的表达有关.