远端缺血预处理对大鼠小体积肝移植术后早期再生的影响

摘要

Objective To investigate the effects of remote ischemic preconditioning(RIPC) on the early liver regeneration after small-for-size liver transplantation in rats. Methods After small-for-size liver transplantation model was established, 48 rats were equally randomized into 2 groups of A (treated with RIPC before liver transplatation) and B (liver transplatation without RIPC). Serum ALT was detected at 2,6,12, 24 hours after operatioa The morphological changes of liver tissues were observed and the Ki-67 expression was measured by immunohistochemistry for evaluating the cell proliferation. The expressions of cyclin D1 mRNA and protein were detected by RT-PCR and Western blot, respectively. Results Compared with group B, serum ALT was decreased, the pathological injury of the liver was less, the positive expression of Ki-67 and the expressions of cyclin Dl mRNA and protein were all increased in group A(P<0. 05). Conclusion RIPC can attenuate early liver injury probably via improving early hepatocyte regeneration after small-for-size liver transplantation in rats.%目的 探讨远端缺血预处理(RIPC)对大鼠小体积肝移植后肝脏早期再生的影响.方法 建立大鼠小体积肝移植模型后,48只SD大鼠随机均分为RIPC(A)组和对照(B)组,分别于移植术后2、6、12、24 h检测血清ALT水平,并采集肝脏组织标本以观察形态学变化.采用免疫组织化学法检测Ki-67表达来评价肝脏细胞增殖情况,RT-PCR和Western blot法分别检测各时间点周期蛋白D1 (cyclin D1) mRNA和蛋白表达.结果 与B组相比,A组各时间点血清ALT水平降低,大鼠肝脏病理损伤减轻,Ki-67阳性细胞数、cyclin D1 mRNA和蛋白表达水平均明显增加(P＜0.05).结论 RIPC可能通过促进肝脏移植后肝细胞早期再生,从而减轻大鼠小体积移植术后早期肝脏损伤.