水通道蛋白在卵巢上皮癌中的表达及意义

摘要

目的 探讨水通道蛋白1、3(AQP1、AQP3)在上皮性卵巢癌中的表达及其意义.方法 采用免疫组织化学方法检测上皮性卵巢良性、交界性、恶性肿瘤中肿瘤及腹膜组织中AQP1和AQP3的表达,同时检测血管内皮生长因子(VEGF)表达并以CD105标记各组织微血管内皮细胞测定微血管密度(MVD).结果 卵巢癌患者腹膜中AQP1、AQP3表达明显高于良性肿瘤者(P＜0.05).与卵巢肿瘤性质有关；卵巢癌患者中,有腹水(尤其是腹水量≥1000 ml)和淋巴结转移者AQP1、AQP3的表达明显高于无腹水者(P＜0.05).AQP3的表达与卵巢癌病理分期分级有关.结论 AQP1、AQP3参与卵巢癌发生发展、侵袭转移和腹水形成.腹膜组织上AQP1、AQP3的高表达与肿瘤盆腔扩散和腹水生成有密切关系.%Objective To study the expression and significance of aquaporin 1 (AQP1) and AQP3 in ovarian cancer. Methods The expressions of AQP1 and AQP3 in epithelial ovarian tumors and peritoneum tissues were detected by immunohistochemical technique and microvessel density (MVD) in endothelium was determined by CD105 labeling. Results The expressions of AQP1 and AQP3 in ovarian malignant and borderline tumors were higher than those in benign tumors (P<0. 05). The expressions of AQP1 and AQP3 were higher in the cases with lymph node metastasis and ascites (especialy ascites volume more than 1000 ml) than those without (P<0. 05). AQP3 expression was associated with pathological grade and stage. Conclusion AQP1 and AQP3 participate in the invasion, metastasis and ascites formation of epithelial ovarian tumor. The high expression of AQP1 and AQP3 in peritoneum are closely related with pelvic metastasis and formation of ascites in the patients with ovarian cancer.