贵阳地区幽门螺杆菌毒力基因型与上消化道疾病的相关性

摘要

目的 研究贵阳地区幽门螺杆菌(HP)中空泡毒素基因( vacA)和毒素相关基因A(cagA)与上消化道疾病间的关系.方法 用特异的16S rRNA PCR进行临床分离HP的菌种鉴定,对经过鉴定的152株HP菌株应用PCR进行vacA及其基因亚型、cagA的检测.结果 152株HP中,cagA和vacA阳性率分别为39.5％和100％,vacA sla-m2组合的基因型明显多于s1a-m1、s1b-m2基因型(76.3％ vs.15.8％、5.3％)(P＜0.05).不同vacA亚型与慢性胃炎、消化性溃疡及胃癌间未见明显相关性(P＞0.05).结论 贵阳地区的HP以cagA阴性、vacA s1a-m2亚型占优势,不同基因型菌株与胃黏膜病变类型无关.%Objective To investigate the association between vacuolating cytotoxin gene(vacA) and cytotoxin-associated gene A(cagA) in Helicobacter pylori(HP) and upper gastrointestinal disease in Guiyang district,China. Methods The HP strains isolated from clinical tissues were identified by PCR of specific 16S rRNA. The vacA and its gene subtypes and cagA of identified 152 HP strains were detected by PCR. Results In 152 HP strains, the positive rates of cagA and vacA were 39. 5% and 100%,and the vacA sla-m2 component genotype was significantly more than sla-m1 and slb-m2 genotypes(76. 3% vs. 15.8% and 5. 3%) (P<0. 05). Different vacA subtypes were not correlated with chronic gastritis,peptic ulcer and gastric cancer(P>0. 05). Conclusion The cagA-negative and vacA sla-m2 subtype of HP are dominant in Guiyang district. There is no association between different genotype strains and pathological changes of gastric mucosa.