PCR 斑点杂交技术在肺炎支原体感染检测中的应用价值

摘要

Objective To investigate the application value of PCR spot hybridization technique in the detection of mycoplasma pneumoniae(MP) infection .Methods The DNA of 70 respiratory tract specimens from the children with MP infection was extracted for MP specific DNA amplification by PCR .The detection efficiency of MP infection was compared between traditional PCR method and PCR spot hybridization technique .Results Of 70 specimens ,32 specimens were MP positive and 28 specimens were MP negative detected by traditional PCR method and PCR spot hybridization technique .The MP positive was detected by PCR spot hybridization technique in 10 specimens ,which were MP negative by traditional PCR method (P<0 .05) .The sensitivity ,specificity and diagnostic coincidence rate of MP detection by PCR spot hybridization technique were 82.5% ,95.6% and 81.4% ,respectively ,which were higher than 69.6% ,80.6% and 50.2% by traditional PCR method . Conclusion Compared to traditional PCR method ,PCR spot hybridization technique has higher sensitivity and specificity in the detection of M P infection .%目的：探讨 PCR斑点杂交技术在检测肺炎支原体（M P ）感染中的临床应用价值。方法收集疑似MP感染患儿的呼吸道标本70份，提取DNA后采用PCR技术将MP特异性DNA片段进行扩增；比较传统PCR法和PCR斑点杂交技术检测M P感染的效能。结果32份临床标本经传统PCR法和PCR斑点杂交技术检测M P均为阳性，28份临床标本检测均为阴性；10份临床标本PCR斑点杂交技术检测为阳性，但传统 PCR法检测为阴性，配对样本χ2检验显示两法检测M P的差异有统计学意义（P＜0．05）。 PCR 斑点杂交技术检测 MP 的灵敏度为82.5％，特异度为95.6％，诊断符合率为81.4％；均高于传统PCR法的69.6％、80.6％和50.2％。结论与传统PCR法比较，PCR斑点杂交技术检测M P的灵敏度和特异度高。