地塞米松对胎盘细胞生长及葡萄糖转运功能的影响

摘要

Objective To investigate the effect of dexamethasone (DEX) on placental cell growth and glucose transport function.Methods A total of 160 women of 32-34 weeks pregnancy with threatened premature labor was randomly and equally divided into two groups.Group D was given DEX 6 rng by intramuscular injection every 12 hours for two days.Group C was not given DEX treatment.Ten placenta samples were randomly selected from two groups after delivery,respectively.The circumference of nuclear and cell apoptosis were detected by HE staining and TUNEL staining in two groups.qRT-PCR and Western blot were used to detect the mRNA and protein expressions of glucocorticoid receptors(GR) α,glucose transporter(GLUT) 1 and GLUT3.Results Compared to group C,group D had longer circumference of nuclear and more cell apoptosis(P＜0.01 or P＜0.05).The mRNA and protein expressions of GRα,GLUT1 and GLUT3 were decreased in group D than those in group C(P＜0.01 or P＜0.05).Conclusion DEX can inhibit the growth of placental cells and decrease the transport efficiency of glucose.%目的 探讨地塞米松(DEX)对胎盘细胞生长及葡萄糖转运功能的影响.方法 孕32～34周的先兆早产孕妇160例,随机均分为DEX组和对照组.DEX组肌肉注射DEX6 mg,每12小时1次,持续2d;对照组不用DEX.两组孕妇分娩后各取10例胎盘组织样本,采用苏木素-伊红(HE)染色和TUNEL染色分别检测两组胎盘组织细胞核周长和细胞凋亡情况,qRT-PCR及Western blot检测两组糖皮质激素受体(GR)α、葡萄糖转运蛋白(GLUT)1和GLUT3的mRNA及蛋白表达.结果 与对照组比较,DEX组的细胞核周长较长(P＜0.01),细胞凋亡增多(P＜0.05).DEX组GRα、GLUT1和GLUT3 mRNA和蛋白表达较对照组减少(P＜0.01或P＜0.05).结论 DEX能够抑制胎盘细胞的生长,降低胎盘对葡萄糖的转运效率.