绵羊肺炎支原体P208蛋白质的分子特征分析及免疫原性

摘要

为了研究绵羊肺炎支原体P208蛋白质的结构及功能,本试验在对p208基因进行生物信息学分析的基础上,对其部分基因片段进行了克隆及原核表达,并采用Western-blot对其免疫原性进行分析.结果显示,p208基因与猪肺炎支原体黏附素p216基因、殊异支原体细胞表面蛋白基因以及絮状支原体P97/LPPS家族蛋白基因具有较高的同源性.重组P208蛋白以可溶性形式在大肠杆菌BL21(DE3)工程菌中成功得到表达;纯化的重组蛋白与山羊抗绵羊肺炎支原体全菌血清发生结合反应,表明P208蛋白质是绵羊肺炎支原体的免疫原之一.研究结果为进一步分析其功能及其应用提供了重要的依据.%To investigate the structure and function of P208 protein of Mycoplasma ovipneumoniae,based on bioin-formatics analysis,a fragment of p208 gene was amplified and expressed in Escherichia coil. The immunogenicity of P208 was analyzed by Western-blot. The results showed that p208 gene was homologous to the p216 in Mycoplasma hyopneumoni-ae,a gene encoding cell surface protein of Mycoplasma dispar and a gene encoding P97/LPPS family protein of Mycoplasma flocculare. The P208 was successfully expressed in E.coil BL21(DE3) with soluble form. The purified recombinant protein could react with goat serum against M. ovipneumoniae,this suggesting that P208 was one of the immunogens of M. ovipneu-moniae. The results provide an important basis for further analysis of the function and application of P208.