狼疮性肾炎患者Th1、Th17及Th22细胞亚群的变化及临床意义

摘要

Objective To investigate the expression of T-helper (Th)1,Th17 and Th22 cells in peripheral blood and cytokines in serum of patients with lupus nephritis (LN) and its clinical significance.Methods The proportion of Th1,Th17 and Th22 cells in peripheral blood of 20 LN patients and 20 healthy controls were evaluated by flow cytometry; the concentration of cytokines including interferon (IFN)-γ,interleukin (IL)-17 and IL-22 were measured by ELISA.The correlation between proportion of Th 17 and Th22 cells and IL-17,IL-22,systemic lupus erythematosus disease activity index (SLEDAI) were analyzed.Results The proportion of Th17 cells increased (P ＜ 0.05),the proportion of Th22 cells decreased (P ＜ 0.05),the proportion of Th 1 cells did not change too much (P ＞ 0.05) in LN patients compared with healthy controls; the concentration of serum IL-17 increased (P ＜ 0.05),the concentration of serum IL-22 decreased (P ＜ 0.05),the concentration of serum IFN-γ did not change too much (P ＞ 0.05) in LN patients compared with healthy controls.The proportion of Thl7 cells correlated positively with concentration of serum IL-17 and SLEDAI in LN patients (r =0.721,P ＜ 0.05; r =0.225,P ＜ 0.05).There was a positive correlation between the proportion of Th22 cells and concentration of serum IL-22 (r =0.489,P ＜ 0.05),no statistical correlation between the proportion of Th22 cells and SLEDAI (r =0.241,P ＞ 0.05).Conclusion The abnormal expression of Th 17 and Th22 may play an important role in LN disease pathogenicity.%目的 研究狼疮性肾炎(LN)患者外周血辅助性T细胞1 (Th1)、辅助性T细胞17(Th17)和辅助性T细胞22(Th22)及其细胞因子干扰素-γ(IFN-γ)、白细胞介素17(IL-17)和白细胞介素22(IL-17)的表达情况及其临床意义.方法 流式细胞术检测20例LN患者组和20例健康对照组外周血中Th1、Th17和Th22细胞比例;酶联免疫吸附试验(ELISA)检测血清中相关细胞因子IFN-γ、IL-17及IL-22水平,并进行相关性分析.结果 相较健康对照组,LN组外周血中Th17细胞比例增高(P＜0.05),Th22细胞比例减低(P＜0.05),而Th1细胞比例无差异(P＞ 0.05);LN组血清中IL-17水平增高(P＜0.05),IL-22水平减低(P＜0.05),而IFN-γ水平无差异(P＜0.05);Th17细胞比例分别与血清IL-17、系统性红斑狼疮疾病活动指数(SLEDAI)呈正相关(r=0.721,P＜0.05; r=0.225,P＜0.05),而Th22细胞比例与血清IL-22水平呈正相关(r=0.489,P＜0.05)与SLEDAI评分无相关性(r=0.241,P＞0.05).结论 Th17及Th22在LN患者中异常表达可能导致患者自身免疫功能紊乱,促进疾病病理损伤.