血管内皮生长因子基因修饰骨髓间充质干细胞移植于放射治疗后组织的实验研究

摘要

Objective This study aimed to resume the ability of revascularization of the irradiated tissue by implantation of vascular endothelial growth factor(VEGF)-modified bone mesenchymal stromal cell(BMSC). Methods Each mouse was irradiated with 60Coγray on their right leg. The irradiated muscular tissues of the animal models were injected with VEGF165-modified BMSCs, and a second dose was administered after two weeks. We determined the expression of VEGF165 mRNA, VEGF165 protein, and ultrastructure of the vessels and nerves after gene therapy. Results VEGF165 mRNA and VEGF165 protein had high levels of expression in the irradiated tissue after the injection of VEGF165 -modified BMSCs. The configuration and ultrastructure of the vessels and nerves returned to normal. Conclusion VEGF165-modified BMSCs can resume the ability of the vessels and nerves of an irradiated tissue. This study provides abasis for the clinical application of VEGF165-modified BMSCs.%目的：采用血管内皮生长因子（VEGF）基因修饰骨髓间充质干细胞（BMSC），观察其是否能改善放射治疗损伤区组织的生长情况。方法以60Coγ射线照射大鼠右后肢，在照射结束后第1天，将VEGF165基因修饰的BMSC注射至照射区肌肉组织细胞，共注射2次，间隔2周，治疗结束后行检测VEGF165 mRNA、VEGF蛋白含量，并观察其血管神经的显微结构。结果注射VEGF165基因修饰的BMSC治疗后，照射区组织中的VEGF165 mRNA及VEGF蛋白的表达均明显增高，血管、神经超微结构基本恢复正常。结论 VEGF165基因修饰的BMSC能有效修复肌肉组织的放射损伤，为临床治疗提供了一定的实验基础。