感觉神经肽P物质对气道平滑肌细胞收缩幅度的影响

摘要

Objective To investigate the effect of neuropeptide substance P on airway smooth muscle cell contraction amplitude.Methods According to random method, 10 Wistar rats were divided into normal group and asthmatic group.By inhaled OVA to make asthmatic rat model;primary culture ASMC;confocal microscopy were used to observe the morphological changes and measure the length before and after different intervention.The percent of contraction length come from different group ASMC were used for statistical analysis.Results The ASMCs volume in acetylcholine intervented group and substance P intervented group decreased significantly,cell diameters shorten, cytoplasm reduction and cell arranged densely.The ASMCs volume in substance P receptor antagonist intervented group and nimodipine intervented group are about the same size as the ones in normal control group, were spindle-shaped, abundant cytoplasm and arrangement regularly.The contraction length percent of Ach intervened group is the biggest(19.60 ± 3.47) ％, contraction length percent of nimodiping intervented group is the shortest(3.25 ± 1.14)％ ,the contraction length percent in substance Precepter antagonist intervented group is bigger than the one in control group (3.54 ± 1.26) ％, but less than the one in Ach intervented group, asthmatic (14.36 ± 2.37) ％ and substance P intervened group (17.79 ± 3.19) ％.Conclusion Substance P can increase the amplitude of airway smooth muscle cell contraction, but the effect less than Ach;substance P receptor antagonists can inhibit smooth muscle cell contractility, but the effect less than nimodipine.Substance P participates in acute attack of asthma, increases airway reactivity by increasing airway smooth muscle contraction intensity.%目的 探讨感觉神经肽P物质对气道平滑肌细胞(airway smooth muscle cell,ASMC)收缩幅度的影响.方法 将10只Wistar大鼠按照随机分组原则分成正常对照组及哮喘模型组,卵蛋白吸入法制作哮喘大鼠模型;原代培养两组大鼠气道平滑肌细胞;激光共聚焦显微镜观察ASMC在不同干预因素干预前后细胞形态及收缩的长度的变化,计算各组细胞收缩百分比,对各组ASMC收缩长度百分比进行统计学分析.结果 乙酰胆碱干预组及P物质干预组ASMC体积明显变小,细胞直径缩短,胞浆减少,细胞排列密集.P物质受体拮抗剂干预组及尼莫地平干预组ASMC细胞体积与正常对照组细胞体积大小相仿,细胞呈梭形,胞浆丰富,细胞排列规则.乙酰胆碱干预组细胞收缩百分比最大(19.60 ±3.47)％,尼莫地平干预组细胞收缩百分比最小(3.25±1.14)％,P物质受体拮抗剂干预组细胞收缩百分比(3.56±1.03)％较正常对照组(3.54±1.26)％大,但较乙酰胆碱干预组(19.60±3.47)％、哮喘组(14.36±2.37)％及P物质干预组(17.79±3.19)％小,差异均有统计学意义(P＜0.05).结论 感觉神经肽P物质会增加ASMC收缩幅度,但其收缩幅度较乙酰胆碱小;其受体拮抗剂有抑制平滑肌细胞收缩能力的作用,但其抑制能力较尼莫地平弱.感觉神经肽P物质通过增加气道平滑肌细胞收缩力参与哮喘急性发作.