低血清浓度下骨形成蛋白2对软骨细胞的作用

摘要

Objective:To investigate the effect of bone morphogenetic proteins 2 (BMP-2) on adhesion and growth of mandibular condylar chondrocytes (MCCs) in SD rats,and explore the optimum concentration of BMP-2.Methods:The MCCs were identified and cultured in low fetal bovine serum (2.5％ FBS);The cells were divided into four groups (n=3):a control group (without BMP-2),a BMP40 group (induced with 40 ng/mL BMP-2),a BMPl00 (induced with 100 ng/mL BMP-2),a BMP200 (induced with 200 ng/mL BMP-2).Methyl thiazolyl tetrazolium (MTT) method was used to evaluate cell proliferation ability at 8 h,12 h,1 d,3 d,5 d,7 d.Results:The absorbance detected by MTT assay showed that BMP100 (0.497±0.010) was significantly higher than that in control group (0.345±0.024),BMP40 group (0.475±0.007) and BMP200 group (0.471±0.013) at 1 d,the difference was statistically significant (P＜0.05).However,the growth curve did not increase geometrically.Conclusion:The optimum concentration of BMP-2 is 100 ng/mL.BMP-2 can effectively promote the initial adhesion of MCCs,but the proliferation effect did not show significantly difference.%目的:研究骨形成蛋白2(bone morphogenetic proteins 2,BMP-2)对SD乳鼠下颌骨髁突软骨细胞(mandibular condylar chondrocytes,MCCs)黏附和增殖作用并探索BMP-2的最佳实验浓度.方法:在低血清浓度(含2.5％胎牛血清培养基)培养条件下,分为4组(n=3):对照组(未添加BMP-2)、BMP40组(添加40 ng/mL的BMP-2)、BMP100组(添加100 ng/mL的BMP-2)、BMP200组(添加200 ng/mL的BMP-2).通过甲基噻唑基四唑(methyl thiazolyl tetrazolium,MTT)比色法测量8h,12h,1d,3d,5d,7d活细胞数并描绘生长曲线.结果:在1d时BMP 100组MTT吸光度(0.497±0.010)较对照组(0.345±0.024)、BMP40组(0.475±0.007)及BMP200组(0.471±0.013)均高,差异具有统计学意义(P＜0.05).细胞数未呈指数增长.结论:低血清浓度下,BMP-2用于MCCs的最佳实验浓度为100 ng/mL,可促进MCCs的初期黏附但增殖作用并不明显.