Objective To establish a method for relatively quantitative measurement of HLA-B27 mRNA.Methods Totally 27 cases of peripheral venous blood samples were collected from patients with ankylosing spondylitis,including 22 cases from the patients with HLA-B27 positive and 5 cases with HLA-B27 negative.Total RNA from the venous blood samples was extracted,and then the expression level of HLA-B27 mRNA was quantitatively detected by using reverse transcription polymerase chain reaction(RT-PCR).Results The expression level of HLA-B27 mRNA was higher in patients with HLA-B27 positive(n=22;1.16-37.01,mean value was 7.77)than that in patients with HLA-B27 negative(n=5;mean value was 1.19,close to that of healthy control group).Conclusion Relatively quantitative measurement of HLA-B27 mRNA is helpful for monitoring of ankylosing spondylitis.%目的 建立检测HLA-B27 mRNA表达水平的相对定量的方法.方法 筛选未经治疗的AS患者外周静脉血27例,其中HLA-B27阳性患者22例,HLA-B27阴性患者5例,对标本进行Total RNA提取.然后用RT-PCR方法进行HLA-B27反转录及相对定量.结果 22例HLA-B27阳性AS患者有着相对较高的HLA-B27 mRNA表达水平,最低值为1.16,最高值为37.01,均值为7.77.5例HLA-B27阴性AS患者均值为1.19,接近于HLA-B27阴性健康对照组.结论 检测外周静脉血HLA-B27 mRNA表达水平对检测AS是一种有效可行的方法.
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