电化学发光免疫分析定量检测血清抗HBs的临床意义

摘要

目的 探讨电化学发光免疫分析技术(ECLIA)定量检测乙型肝炎病毒表面抗体(抗-HBs)的临床意义.方法 用ECLIA和酶联免疫吸附法(ELISA)对质控品和680例临床血清标本同时进行测定,对两种方法的结果经χ2检验,进行比较分析.结果 ECLIA检测灵敏度为2.0 mIU/mL,ELISA法灵敏度为20 mIU/mL,两种检测方法检测103例正规乙肝疫苗接种患者抗-HBs的阳性检出率分别为ECLIA 83.5%、ELISA 60.1%;对已确诊肝炎患者577例的标本检测,ELISA法漏检率为4.3%;对溶血标本的测定ECLIA法优于ELISA法.结论 ECLIA是目前较好的定量检测抗-HBs的新技术,在乙型肝炎的诊断、疗效观察和预后判断等方面有重要的临床意义.%Objective To investigate the clinical significance of quantitative detection of hepatitis B virus surface antibody by electrochemiluminescence immunosorbent assay (ECLIA).Methods The quality control preparation and 680 cases of clinical serum samples were determined by applying both ECLIA and enzyme immunoassay (ELISA) method simultaneously, and then χ2 test was adopted to perform comparative analysis on the above results.Results The sensitivity of ECLIA and ELISA was 2.0 mIU/mL and 20 mIU/mL, respectively. The anti-HBs positive detection rate of ECLIA and ELISA was 83.5% and 60.1% respectively in 103 subjects undergoing hepatitis B vaccine inoculation. The omission rate of ELISA detection was 4.3% for the serum samples of definitely-diagnosed patients with hepatitis B. ECLIA was prior to ELISA for measurement of hemolytic samples.Conclusion ECLIA is at present a relatively good technique on anti-HBs quantitative detection. It displays extra importance in the diagnosis, therapeutic effect observation and prognosis evaluation of hepatitis B.