抗结缔组织生长因子小分子干扰RNA对大鼠肝纤维化的影响

摘要

Objective To explore the effect of small interfering RNA targeting connective tissue growth factor( CTGF siRNA)on liver function and fibre index of rats with liver fibrosis. Methods Thirty male rats were randomly divided into five groups,including control group,model group, prevention group, curative group and advanced curative group. All groups were detected for liver function and liver fibrosis. Results Compared with model group, liver tissue inflammation,necrosis and fibrosis prevention and curative groups significantly reduced , serum ALT , AST, total bilirubin , index of liver fibrosis were significantiy lower and albumin significantly increased (P＜O. 05). Of Advanced curative group, compared with model group, the liver inflammation . necrosis and fibrosis reduced , serum ALT , AST, total bilirubin and liver fibrosis decreased , and albumin increased ( P＜O. 05 ). Conclusion The tail intravenous infusion of CTGF siRNA could effectively control rat liver fibrosis,improve liver function.and have certain curative effect for rats with liver fibrosis. CTGF resistance against hepatic fibrosis siRNA would become a new treatment strategy%目的 观察抗结缔组织生长因子小分子干扰RNA(CTGF siRNA)对大鼠肝纤维化肝脏功能及肝纤维化指标的影响.方法 雄性SD大鼠30只,随机分成5组,即空白对照组、模型组、治疗组、预防组及晚期治疗组,检测各组肝功能及肝纤维化指标,并对肝脏进行组织学检测.结果 与模型组相比,预防组及治疗组肝组织炎症、坏死及纤维化明显减轻,血清氨基转移酶、总胆红素、肝纤维化指标水平降低,清蛋白升高(P<0.05).晚期治疗组与模型组相比肝组织炎症、坏死及纤维化减轻,血清、总胆红素、肝纤维化指标水平降低,清蛋白升高(P<0.05).结论 经尾静脉注射抗CTGF siRNA可有效防治大鼠肝纤维化,改善大鼠肝脏功能,且对已发生的肝纤维化也具有一定的疗效,提示抗CTGF siRNA将成为抗肝纤维化治疗的新策略.