目的 用SELEX技术筛选结核杆菌特异性分子的标志物的适体,建立酶联寡核甘酸吸附试验方法检测结核菌SELEX适配子抗体,探讨SELEX适配子抗体检测在结核病早期诊断中的应用价值.方法 将临床确诊的肺结核病483例与对照组446例标本同步接受结核杆菌SELEX适配子IgG抗体寡核苷酸酶联免疫法检测,将测定结果与金标判定的结果进行比较.结果 活动性结核患者用寡核苷酸酶联免疫法检测SELEX适配子抗体总的敏感度为59.6%,其中菌阳肺结核敏感度为83.8%,菌阴肺结核敏感度为48.1%,肺外结核的敏感度为64.7%(P＜0.05),诊断结核病的总的特异度为94.4%,在非结核呼吸系统疾病中特异度为95.2%,在健康人群组中特异度为94.0%.结论 酶联寡核苷酸吸附试验检测法操作简便、快速,灵敏性高、特异性好,对结核病的早期诊断有着较高的参考价值.%Objective To screen the typical aptamer of distinctiveness of tubercle bacillus though SELEX (systematic evolution of ligands by exponential enrichment aptamers IgG antibodies) technology;To establish the enzyme-linked oligonucleotide assay to test the typical aptamer antibody;To explore the application value of SELEX technology in aptamer antibody testing in early diagnosis of tuberculosis. Methods 483 cases clinically diagnosed as tuberculosis patients were compared and contrasted with 446 cases on trial. ELISA(Enzyme-linked immunosorbent assay) was performed synchronically on the aptamer antibody tested by SELEX technology from these cases. The results were compared with those from gold standard. Results The total sensitivity of active pulmonary tuberculosis though immune method was 59. 6% , among which positive tuberculosis sensitivity was 83. 8% , negative tuberculosis sensitivity was 48. 1% , extra-pulmonary tuberculosis patients was 64. 7%(P<0. 05). The specificity of the tuberculosis patients and no-tuberculosis were 94. 4% and 95. 2% ,the specificity of the healthy group was 94. 4%. Conclusion Enzyme-linked oligonucleotide assay detection method is simple,rapid,of high sensitivity and specificity,and of high reference value for early diagnosis of tuberculosis.
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