特发性肺纤维化患者血管紧张素转换酶基因多态性与其血清浓度的关系

摘要

Objective To study the; association between angiotensin converting enzyme (ACE) gene polymorphism and ACE activity in idiopathic pulmonary fibrosis patients .Methods ACE genotype of I/D was determined by polymerase chain reaction (PCR ),and the ACE level of the serum was measured by ELISA method .Results Compared with control group,the serum ACE level of the IPF patients was decreased significantly (P<0.05),ACE gene polymorphism was associated with the ACE activity of the serum,the highest serum ACE activity was in genotype II followed by genotype ID and gene DD .Conclusion ACE gene polymorphism is important factor to determine the ACE activity in idiopathic pulmonary fibrosis patients .%目的 探讨特发性肺纤维化(IPF)患者血管紧张素转换酶(ACE)基因多态性与其血清ACE浓度的关系.方法 采用聚合酶链反应(PCR)技术检测ACE基因I/D多态性,应用ELISA法测定血清ACE的浓度.结果 IPF患者的血清ACE浓度与对照组比较明显降低,差异有统计学意义(P＜0.05),其基因多态性与血清ACE水平有关,ACE水平由高到低依次为:II基因型、ID基因型、DD基因型.结论 ACE基因多态性是决定IPF患者血清ACE水平的重要因素.