凝固酶阴性葡萄球菌的鉴定及分类

摘要

目的 探讨凝固酶阴性葡萄球菌简化生化反应检索表的鉴定分类技术.方法 采用凝固酶活性,溶血作用,硝酸盐还原以及有氧条件下分解,下记10种糖类(木糖、蔗糖、阿拉伯糖、海藻糖、木糖醇、果糖、麦芽糖、乳糖、核糖、甘露醇)等实验进行鉴定分类.结果 按简化生化反应检索表的程序对446株凝固酶阴性葡萄球菌鉴定分类,获得表皮葡萄球菌322株(72.2%),孔氏葡萄球菌36株(8.1%),木糖葡萄球菌26株(5.8%),华氏葡萄球菌24株(5.4%),腐生葡萄球菌20株(4.5%),头状葡萄球菌4株(0.9%),人型葡萄球菌及溶血葡萄球菌各2株,另有10株不能定种.结论 凝固酶阴性葡萄球菌按凝固酶实验和甘露醇实验的结果可初步分为表皮葡萄球菌和其他凝固酶阴性葡萄球菌两大类,在观察有氧条件下各种糖类分解实验过程中,注意到凝固酶阴性葡萄球菌对多种糖类能在培养48 h内分解产酸,但对木糖和麦芽糖的分解,有些菌种则需3～7 d,呈迟缓分解.建议糖类分解实验以连续观察7 d再确定阴性为好.%Objective To explore the appraisal and classification for the simple biochemistry reaction research form of the con densed enzyme negative Staphylococcus. Methods Use condensed enzyme active,hemolysis function,muriatic acid reduction and de composition under the oxygen and 10 categories as follows(xylose, sugarcane sugar, Arabic sugar,seaweed sugar, xylose mellow, fructose maltose,malt sugar ,lactobiose, ribose,mamaitol) and so on as testing, appraisal and classification. Results 446 plants of condensed enzyme negative staphylococcus are appraised for the classification to obtain 322 plants of surface staphylococcus (72. 2%) ,36 plants of KongShi Staphylococcus(8. 1 %) ,26 plants of xylose Staphylococcus (5. 8%) ,24 plains of Fahrenheit Staph ylococcus (5. 4%) ,20 plants of saprophytic Staphylococcus(4. 5 %) ,4 plants of head status Staphylococcus(0. 9%) ,2 plants of man type of Staphylococcus and hemolysis Staphylococcus separately and other 10 plants that couldn t be definite in the variety. Conclusion The condensed enzyme negative staphylococcus could be initially classified into the surface Staphylococcus and other con densed enzyme negative Staphylococcus as two major categories in accordance with the result of the condensed enzyme and mannitol tesring. The condensed enzyme negative Staphylococcus could be paid more attention to decomposing to a variety of the sugar types to produce the acid within 48 hours in the course of a variety of sugars decomposing testing under the oxygen in the observation. However,the xylose and malt sugar decomposing needs 3 - 7 days to show delaying decomposing in the certain bacterial categories. Suggestion,the sugar decomposing testing is better for the decision of the negative after continuing 7 days testing.