低水平 HBsAg 标本的检测及其临床意义

摘要

目的：探讨低水平乙型肝炎病毒表面抗原（HBsAg）的检测方法及其临床意义。方法血清样本为上海科华公司生产的 HBsAg 酶联免疫吸附试验（ELISA）试剂盒或罗氏 cobas E601分析仪免疫发光分析仪初筛为弱阳性的标本；用丽珠 HBsAg中和试剂盒（酶联免疫法）或罗氏 cobas E601进行确认试验，并结合相应样本的肝功能及 HBV-DNA 检测结果进行分析。结果经科华试剂盒初筛为弱阳性的53例样本中，丽珠 HBsAg 中和试剂盒检测为阳性37例、阴性16例；经电化学发光法检测为弱阳性的18例样本中，丽珠 HBsAg 中和试剂盒检测为阳性4例，阴性6例。结论不同检测系统检测低水平 HBsAg 的结果存在差异，低水平 HBsAg 的临床价值有待进一步研究。%Objective To investigate the methods for the detection of serum samples with low-concentration HBsAg and its clin-ical value.Methods Serum samples with HBsAg weak positive were measured by ELISA or using Roch cobas e601 were collected from the clinical laboratory.The samples with low-concentration HBsAg were confirmed by neutralization assay by using Lizhu ELISA reagent Kit or Roch cobas e601 analyzer.at the same time,ALT/AST concentrations and HBV DNA copies were also deter-mined.Results Among 53 samples that showed weak positive reaction by using Kehua reagent kit,37 samples were positive in neu-tralization test,while the other 16 samples were negative.Among 18 samples that showed weak positive reaction tested by using Roch cobas e601 analyzer,13 samples showed positive in neutralization test,and the other 5 samples showed negative.Conclusion The results of HBsAg test varies in different detection System.The clinical significance of samples with low-concentration HBsAg needs further study.