乙型肝炎病毒感染相关疾病患者 T 淋巴细胞亚群的变化与意义

摘要

Objective To investigate clinical significance of the changes in the peripheral blood T lymphocytes subsets in patients with diseases related to HBV infection .Methods 257 cases of inpatients and outpatients were selected from Jan .to Dec .2013 ,and were divided into hepatitis B carriers (ASC)group ,chronic hepatitis B(CHB)group ,hepatocirrhosis(LC)group and primary liver cancer(PHC)group according to types of diseases related to HBV infection .Other 50 healthy individuals conducted physical exami‐nation were enro1led in the control group .The absolute CD3+ ,CD4+ and CD8+ T‐cell count ,and CD3+ ,CD4+ and CD8+ percent‐age and CD4+ /CD8+ value were detected in all subjects by using flow cytometer .These data were compared and analyzed .Results Compared with the control group ,there were no significant differences of the absolute CD3+ ,CD4+ and CD8+ T‐cell count ,and CD3+ ,CD4+ and CD8+ percentage and CD4+ /CD8+ value in ASC group ,CHB group and LC group(P>0 .05) .Compared with the control group ,the absolute CD4+ T‐cell count ,CD4+ percentage and the CD4+ /CD8+ value were decreased in the PHC group , while the CD8+ percentage were increased in the PHC group ,there were statistical significant differences between the two groups (P<0 .05) .Conclusion The peripheral blood T lymphocyte subsets could be monitor indexes of cell immune function in diseases related to HBV infection .%目的：探讨乙型肝炎病毒（HBV ）感染相关疾病患者淋巴细胞亚群变化的临床意义。方法选取2013年1～12月住院和门诊患者257例，根据 HBV感染相关疾病类型分为乙肝携带者（ASC）组、慢性乙型肝炎（CHB）组、肝硬化（LC）组、原发性肝癌（PHC）组；另选取同期体检健康者50例纳入对照组。采用流式细胞仪检测所有受试者外周血CD3＋、CD4＋、CD8＋ T淋巴细胞绝对值和百分比，以及CD4＋／CD8＋比值，并进行比较分析。结果 ASC、CHB、LC组患者CD3＋、CD4＋、CD8＋ T淋巴细胞绝对值和百分比，以及CD4＋／CD8＋比值与对照组比较差异均无统计学意义（P＞0．05）；与对照组比较，PHC组患者CD4＋ T淋巴细胞绝对值和百分比、CD4＋／CD8＋比值均降低，CD8＋ T淋巴细胞百分比升高，差异均有统计学意义（ P＜0．05）。结论外周血淋巴细胞亚群可作为HBV慢性感染患者细胞免疫功能的监测指标。