Objective To investigate the genetic location of SCCmec-associated psm-mec in Staphylococcus hominis isolated from blood culture,and to lay a foundation for further functional studies of psm-mec in Staphylococcus hominis.Methods 25 strains of Staphylococcus hominis isolated from positive blood culture were collected.mecA and psm-mec gene were amplified by PCR,and the SCCmec types were determined by the results of multiplex PCR assay.For analyzing the genetic location characteristic of psm-mec in SCCmec,three pair special PCR primers were used to measure mecR1/psm-mec,psm-mec/xylR and fudoh respectively.Results There were 21 strains of methicillin-resistant Staphylococcus hominis and 4 strains of methicillin-sensitive Staphylococcus hominis. The positive rate of psm-mec gene in methicillin-resistant Staphylococcus hominis was 47.6%,and no psm-mec gene was found in methicillin-sensitive Staphylococcus hominis.Among psm-mec positive strains,2 strains belonged to SCCmecⅢ,5 strains belonged to SCCmecⅢ-like,and 3 strains belonged to new SCCmec types.All of the 10 psm-mec positive strains were mecR1/psm-mec,psm-mec/xylR and fudoh gene positive.Conclusion SCCmec-associated psm-mec extensively exists in methicillin-resistant Staphylococ-cus hominis isolated from positive blood culture,which distributes mainly in typical SCCmecⅢ,SCCmecⅢ-like and new SCCmec types and locates between mecR1 and xylR gene.%目的：了解 SCCmec 相关的 psm-mec 基因在血液来源人葡萄球菌中的基因定位特征，为深入研究 psm-mec 基因在人葡萄球菌中的功能奠定基础。方法收集临床血培养分离的人葡萄球菌25株，通过 PCR 扩增 mecA 基因和 psm-mec 基因，多重 PCR 对耐甲氧西林人葡萄球菌 SCCmec 分型，PCR 扩增 mecR1／psm-mec 基因与 psm-mec／xylR 基因间隔序列及 fudoh 基因，探究 psm-mec 基因在 SCCmec 上的定位特点。结果 PCR 检测显示，21株为耐甲氧西林人葡萄球菌，psm-mec 基因的检出率为47．6％，4株为甲氧西林敏感人葡萄球菌，未检出 psm-mec 基因。多重 PCR 结果显示，10株 psm-mec 基因阳性菌株中，2株属典型 SCCmec Ⅲ型，5株属类 SCCmec Ⅲ型，3株属 SCCmec 新型别。基因间隔序列和 fudoh 基因检测显示，10株 psm-mec 基因阳性人葡萄球菌中，mecR1／psm-mec 基因和 psm-mec／xylR 基因及 fudoh 基因均阳性。结论 SCCmec 相关的 psm-mec 基因广泛存在于血液来源的人葡萄球菌中，主要分布在典型 SCCmec Ⅲ型、类 SCCmecⅢ型和 SCCmec 新型别上，定位于 mecR1基因与 xylR 基因之间。
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