Sysmex XN-1000血细胞分析仪计数低值白细胞性能的评估

摘要

目的：探讨 Sysmex XN‐1000（后简称 XN‐1000）全自动血细胞分析仪检测白细胞计数（WBC）及分类的准确性。方法收集该院2015年1～4月门诊及住院患者及健康体检者共206例，根据人工镜检检测 WBC 结果分为高值组（WBC ＞10．0×109／L），健康组（4．0×109／L ＜ WBC ≤10．0×109／L）和低值组（1．5×109／L ＜ WBC ≤4．0×109／L）。再分别用 XN‐1000与Sysmex XE‐5000（后简称 XE‐5000）进行 WBC 并分类。采用统计学软件对3种方法的结果进行对比分析，并进行相关性分析。结果3种方法检测 WBC 结果比较，高值组和健康组差异无统计学意义（P＞0．05），结果具有正相关性。低值组中，XN‐1000检测结果准确性与 XE‐5000差异无统计学意义（P＞0．05），但是白细胞分类结果显著高于 XE‐5000（P＜0．05）；对于异常细胞，XN‐1000和 XE‐5000不能进行有效分类，仍需人工镜检进行分类。结论 XN‐1000全自动血细胞分析仪在 WBC 与 XE‐5000及人工镜检比较无差异统计学意义，在测定白细胞分类上 XN‐1000较 XE‐5000更准确（XN‐1000合格率91％，XE‐5000合格率63％），但仍不能完全替代人工镜检。对于异常细胞，XN‐1000不能有效分类，需与人工镜检结合。%Objective To investigate the accuracy of XN‐1000 automatic blood cell analyzer in detecting of white blood cell (WBC) count and classification .Methods A total of 206 cases including outpatients ,inpatients and health people were collected in the hospital from January to April 2015 .According to manual microscopy detection of white blood cell count results ,the cases were divided into three groups :high value of white blood cell(WBC was more than 10 .0 × 109 /L) group ,normal value of white blood cells group(WBC was more than 4 .0 × 109 /L but less than 10 .0 × 109 /L) and low value of white blood cell(WBC was more than 1 .5 × 109 /L but less than 4 .0 × 109 /L) group .WBC count and classification were detected with Sysmex XN‐1000 and Sysmex XE‐5000 respectively .The results and the correlation analysis of the three methods was compared by the statistical software .Results The difference of WBC count between between high value group and normal value group was not statistically significant(P> 0 .05) , and the results detected by Sysmex XN‐1000 was positively correlated with that of Sysmex XE‐5000 and manual microscopy detec‐tion .In low value group ,the accuracy of the test results between Sysmex XN‐1000 and Sysmex XE‐5000 had no significant differ‐ence(P> 0 .05) .However ,the accuracy of the WBC classification by Sysmex XN‐1000(91% ) was significantly higher than Sysmex XE‐5000(63% ,P< 0 .05) .Sysmex XN‐1000 and XE‐5000 couldn′t classify abnormal cells effectively .The classification abnormal cells still needed to artificial microscopy classification .Conclusion The classification of WBC by Sysmex XN‐1000 is more accurate than XE‐5000 ,but it still can not completely replace the manual microscopy ,especially for abnormal cells .The classification of ab‐normal cells should combine the manual microscopy .