阿霉素诱导大鼠心力衰竭模型中血浆NGF表达的初步研究

摘要

Objective To investigate plasma NGF expression in adriamycin induced rat heart failure (HF) model .Methods Twenty‐five Wistar rats were randomly divided into the CHF group (n=15 ,adriamycin 4 mg/kg ,by intraperitoneal injection ,for 6 weeks) and normal control group (NC group ,n=10) ,after successful model construction ,the 6‐week observation was continuously conducted .The body mass and plasma NGF expression were detected once per 2 weeks .Results After 6 weeks later ,the body mass in the CHF group was significantly reduced ,the difference was statistically significant (P<0 .05) ,LVEDV and LVESV were sig‐nificantly increased ,while LVEF was declined obviously (P<0 .05) ,the NGF expression amount was significantly decreased com‐pared with the control group ,the differences were statistically significant (P<0 .05);the NGF expression amount was gradually re‐duced with the time extension of disease course(P<0 .05) .Conclusion Intraperitoneal injection of adriamycin can successfully in‐duce heart failure model in Wistar rats ,moreover NGF may be closely associated with HF .%目的：探讨阿霉素诱导的大鼠心力衰竭模型中血浆神经生长因子（NGF）的表达情况。方法25只Wistar大鼠随机分为心力衰竭组（CHF组，n＝15，阿霉素4 mg／kg ，腹腔注射，6周）和正常对照组（NC组，n＝10），待造模成功后继续观察6周，在6周和12周测定心功能；每2周测定体质量和通过尾静脉采血测定大鼠血浆NGF表达。结果6周后，CHF组大鼠体质量较NC组明显减轻，差异有统计学意义（P＜0．05），LVEDV 和LVESV明显升高，LVEF明显下降，NGF表达量也较对照组明显下降，差异有统计学意义（P＜0．05）；NGF表达量随着病程时间延长而逐渐降低（P＜0．05）。结论 Wistar大鼠腹腔注射阿霉素可以成功诱导HF ，而且NGF可能与HF密切相关。