13442例儿童末梢血白细胞及五分类参数参考范围调查

摘要

目的：对13442例健康儿童末梢血白细胞及五分类参数参考范围调查分析，为临床诊断提供参考。方法按照健康儿童筛选标准及血液疾病、感染性疾病、服药史等排除标准，选择来自广州市妇女儿童医疗中心保健科进行体检和相关检查均正常的健康者，年龄1个月至7岁的儿童13442例，采用日本Sysmex XS‐800i型全自动血细胞分析仪及配套试剂进行白细胞分析参数检测。结果白细胞及五分类参数均不符合正态分布；经 Mann‐Whitney 检验，不同性别儿童间比较，WBC、BASO ＃、NEU＃参数差异无统计学意义（P＞0．05），而LYM＃、MONO＃、EOS＃参数差异有统计学意义（P＜0．001），其中LYM＃女性显著高于男性，MONO＃和EOS＃男性显著高于女性；经Kruskal‐Wallis检验，不同年龄组进行比较，白细胞及五分类参数差异均有统计学意义（P＜0．001），其中WBC、LYM ＃、MONO＃、EOS＃参数随年龄的增加而降低，NEU＃随年龄的增加而不断提高，而BASO＃在4岁前随年龄的增加而下降，4岁后随年龄的增加而增加。结论应该在充分考虑性别、年龄等因素的基础上制定1个月至7岁儿童独立的白细胞类参数的参考值范围。%Objective To investigate the reference ranges of peripheral white blood cells (WBC) and five classification parame‐ters among 13 442 apparently healthy children to provide reference for clinical diagnosis .Methods A total of 13 442 apparently healthy children aged from 1 month old to 5 years undergoing the health physical examination in the healthcare department of the Guangzhou Municipal Women and Children Medical Center were chosen according to the healthy children screening standard and the exclusion standard of hematological diseases ,infection diseases and drug administration history .The WBC analysis and its parame‐ters detection were performed by using the Sysmex XS‐800i automatic blood cell analyzer .Results WBC and its five classification parameters were not accorded with the normally distribution .By the Mann‐whitney test ,no statistically significant differences in WBC ,BASO# and NEU # were detected between different genders of children (P> 0 .05) ,while the differences in LYM # , MONO# and EOS# had statistical significance(P<0 .001) ,in which the LYM # level in females was significantly higher than that in males ,while the MONO# and EOS# levels in females were lower than those in males ;By the Kruskal‐Wallis test ,WBC and five classification parameters had statistically significant differences among different age groups (P<0 .001) .The parameters of WBC ,LYM # ,MONO# and EOS# were decreased with age increase ,while NEU # was constantly increased with age increase and BASO# was decreased with age increase before 4 years old .Conclusion All factors such as gender ,age should be The inde‐pendent reference rangesl of WBC parameters among children aged from 1 month old to 7 years old should be formulated on the ba‐sis of fully considering the factors such as gender ,age .