首页> 中文期刊> 《国际检验医学杂志》 >罗氏生化分析仪上30个检验项目对总胆汁酸测定的干扰因素分析及解决措施

罗氏生化分析仪上30个检验项目对总胆汁酸测定的干扰因素分析及解决措施

         

摘要

Objective To explore the interference factors of total bile acid project and 30 routine items on the Roche Modular P800 automatic biochemical analyzer,and to find the effective measures to eliminate the carry-over.Methods In accordance with the screening process,the interference factors of total bile acid project as the test item,30 regular testing projects as the observation objects,including total bilirubin,direct bilirubin and so on,and then the validation test were carried out.By means of the increasing cleaning procedures of the reagent needles or colorimetric cups and adjusting the test sequence,we compared the influence of the result of total bile acid before and after this kind of treatment.Results There were the carry-over of high density lipoprotein,uric acid and total bile acid,which could be eliminated by increasing cleaning procedures of reagent needles or colorimetric cups.There were the cross contamination of lipase and total bile acid,which could be basically eliminated by adjusting the test sequence.Conclusion There are the cross contamination among the various projects of automatic biochemical analyzer.To avoid this carry-over,we should pay attention to the order of the infectant and contaminated items in setting the analysis parameters,insert several projects in the interval,or add the cleaning procedures of the reagent needles or colorimetric cups with alkali or acid.%目的 探讨Roche Modular P800 全自动生化分析仪上30个常用检验项目对总胆汁酸测定结果的影响,寻找消除携带污染的有效措施.方法 以总胆汁酸为检测项目,以总胆红素、直接胆红素等30个常规检测项目为观察项目,根据交叉污染项目的筛选程序从30个常规项目中筛查出对总胆汁酸测定有干扰的项目,并进行确认试验.对存在携带污染的项目之间增加试剂针、比色杯特殊清洗程序和调整检验顺序,观察经特殊处理前后对总胆汁酸检测结果的影响程度.结果 高密度脂蛋白、尿酸、脂肪酶这3个检验项目对总胆汁酸的测定有干扰;增加试剂针和比色杯的特殊冲洗后,可消除高密度脂蛋白和尿酸对总胆汁酸测定结果的干扰,调整脂肪酶检测顺序后,能基本消除脂肪酶对总胆汁酸结果的干扰.结论 全自动生化分析仪各项目之间存在携带污染,在设定分析参数时,应注意污染和被污染项目检测的顺序,应在两者中间间隔开几个项目,或者用碱液或酸液加强试剂针和反应杯的清洗,从而避免交叉污染的产生.

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