乳腺肿瘤患者外周血T淋巴细胞亚群分析及其临床价值

摘要

目的 检测乳腺癌患者外周血T淋巴细胞亚群及NK细胞表达情况,分析乳腺癌患者细胞免疫功能状况及其临床价值.方法 选择德州市第二人民医院收治的138例乳腺癌患者作为乳腺癌组,再选择50例健康女性作为对照组,采用流式细胞技术检测两组受试者外周血 T 淋巴细胞亚群以及NK细胞水平.结果 与对照组比较,乳腺癌患者CD3+、CD4+、CD4+/CD8+以及 NK 细胞均显著降低,差异有统计学意义(P<0.05),而CD8+细胞水平显著升高,差异有统计学意义(P<0.05);不同临床分期乳腺癌患者细胞免疫功能差异有统计学意义(P<0.05),随着临床分期的升高,患者CD3+、CD4+、CD4+/CD8+以及N K细胞水平降低(P<0.05),而CD8+细胞水平逐渐升高(P<0.05);有淋巴结转移乳腺癌患者CD3+、CD4+、CD4+/CD8+以及NK细胞百分比较无淋巴结转移患者显著降低(P<0.05),而CD8+细胞百分比较无淋巴结转移患者显著升高(P<0.05).结论 乳腺癌患者存在广泛的免疫功能低下或免疫失调,且患者免疫功能的变化与肿瘤进展、淋巴结转移有关,监测患者外周血淋巴细胞亚群水平,对判断乳腺癌患者病情发展、治疗预后具有一定的参考价值.%Objective To detect the expression of T lymphocyte subsets and NK cells in peripheral blood of patients with breast cancer,and to investigate the status of cellular immune function and its clinical value in patients with breast cancer.Methods 138 patients with breast cancer were selected from Dezhou Second Peo-ple′s Hospital,and 50 healthy women were selected as the control group.The peripheral blood T lymphocyte subsets and NK cell activity were detected by flow cytometry in the two groups.Results Compared with the control group,the CD3+,CD4+,CD4+/CD8+and NK cells in breast cancer patients were significantly lower (P<0.05),while the number of CD8+cells increased significantly,and the difference was statistically signifi-cant (P<0.05).The difference of cellular immune function of patients with breast cancer in different clinical stages was statistically significant (P<0.05);with the increase of clinical stages,the numbers of CD3+,CD4, CD4+/CD8+and NK cells in the patients gradually decreased (P<0.05),while CD8+cells increased gradually (P<0.05);the number of CD3+,CD4+,CD4+/CD8+and NK cells in breast cancer patients with lymph node metastasis was significantly lower than that in patients without lymph node metastasis (P<0.05),while the number of CD8+cells was significantly higher than that in patients without lymphatic metastasis(P<0.05). Conclusion Immunodeficiency or immune disorder is very common in breast cancer patients,and changes of immune function in patients are correlated with tumor progression,lymph node metastasis,monitoring the lev-el of peripheral blood lymphocyte subsets in patients with breast cancer has a certain reference value for jud-ging the development of the patient′s condition and the prognosis..